Affinity purified goat anti-rabbit IgG (H&L) antibody is conjugated to horseradish peroxidase.
Applications
This product has been optimized for use as a secondary antibody in chemiluminescent Western blotting applications.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
注意事项：
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol.
Store at –20°C.
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