SignalSilence? WFS1 siRNA I

• 产品编号：CST-7044S      品牌：CST       原厂货号：7044S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence® WFS1 siRNA I抑制人、小鼠和猴子WFS1的表达。CST SignalSilence®WFS1 siRNA I允许研究者使用RNA干扰技术特定地抑制WFS1表达，使用这种方法可以将双链RNA分子转至细胞内，选择性沉默基因的表达。所有CST SignalSilence® siRNA产品都经过严格的内部测试，并用western方法检测证明可以降低靶蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后，该寡核苷酸通过亲和固相萃取纯化。退火的RNA双链进一步通过质谱法进行分析，验证双链的确切成分。通过质谱，将每批结果与之前的批次比较，以确保批次间最大的一致性。CST建议在细胞裂解前48-72h，用100 NM SignalSilence®WFS1siRNA I进行转染。转染程序参照转染试剂制造商所提供的操作指南。在使用上有任何问题，请随时联系CST。Wolfram综合症蛋白(WFS1)是一种890个氨基酸的蛋白质，包含一个细胞质N末端结构域，之后是9个跨膜结构域和一个细胞内的C端区域。 WFS1主要定位于内质网（ER）（1），该蛋白的表达是响应ER应激而产生的，部分是通过转录激活（2,3）。调查研究表明WFS1基因突变会导致Wolfram综合征，该病为常染色体隐性遗传的神经退行性疾病，表现为年轻发病、非免疫性、胰岛素依赖型糖尿病和渐进性视神经萎缩（4）。

1. Takeda, K. et al. (2001) Hum Mol Genet 10, 477-84.
2. Yamaguchi, S. et al. (2004) Biochem Biophys Res Commun 325, 250-6.
3. Ueda, K. et al. (2005) Eur J Endocrinol 153, 167-76.
4. Inoue, H. et al. (1998) Nat Genet 20, 143-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Monkey

Specificity / Sensitivity

SignalSilence® WFS1 siRNA I inhibits human, mouse, and monkey WFS1 expression.

Description

SignalSilence® WFS1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit WFS1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® WFS1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Wolfram syndrome protein (WFS1) is an 890 amino acid protein that contains a cytoplasmic N-terminal domain, followed by nine-transmembrane domains and a luminal C-terminal domain. WFS1 is predominantly localized to the endoplasmic reticulum (ER) (1) and its expression is induced in response to ER stress, partially through transcriptional activation (2,3). Research studies have shown that mutations in the WFS1 gene lead to Wolfram syndrome, an autosomal recessive neurodegenerative disorder defined by young-onset, non-immune, insulin-dependent diabetes mellitus and progressive optic atrophy (4).

1. Takeda, K. et al. (2001) Hum Mol Genet 10, 477-84.
2. Yamaguchi, S. et al. (2004) Biochem Biophys Res Commun 325, 250-6.
3. Ueda, K. et al. (2005) Eur J Endocrinol 153, 167-76.
4. Inoue, H. et al. (1998) Nat Genet 20, 143-8.

注意事项：

Storage: WFS1 siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.