TMB Substrate

• 产品编号：CST-7004S      品牌：CST       原厂货号：7004S
• 产品分类：生化试剂 > 免疫分析试剂 > Luminex缓冲液和试剂
• 应用分类：

描述：

TMB底物可以直接用于ELISA检测中。底物和固定的辣根过氧化物酶（HRP）结合的二抗之间的反应生成蓝色的溶液。颜色的深度和生成时间因其敏感性和实验条件不同而出现差异。达到所需要的颜色深度后，加入酸性终止液可以使反应溶液的颜色右蓝色变为黄色。终止反应1小时内，结果可以保持稳定。板子应该在尽快用读板机于450nm读取并分析结果。TMB具有光敏感性，因此装于琥珀色瓶中，以保护溶液受太阳光直接照射。建议保存在4度。

a）与辣根过氧化物酶（HRP）结合的二抗一起孵育后，用含有0.05%的Tween-20的PBS洗板。 （b）每孔加入100ulTMB溶液。孵育特定的时间或者直到产生所需要的信号。请参考说明书中建议的孵育时间。 （c）加入100ul终止液 （d）在450nm处读取吸光值

TMB（ 3,3‘,5,5’-四甲基联苯胺）在ELISA中作为底物以产生可检测光。TMB与过氧化物酶，典型的是辣根过氧化物酶（HRP）之间的反应可以产生可测量的与分析物相关颜色变化。

原厂资料：

Description

TMB Substrate used is ready to use for ELISA detection. Reaction between the substrate and immobilized horseradish peroxidase (HRP) conjugated secondary antibodies in the ELISA wells produces a blue colored solution. Color intensity and development time will vary depending on assay sensitivity and conditions. After reaching the desired color intensity, the reaction is terminated by addition of an acidic STOP solution which changes the solution color from blue to yellow. While the results will remain stable for one hour following termination, the plate should be analyzed promptly on a microwell reader at 450 nm. TMB is light sensitive and is therefore packaged in amber bottles to protect the solution from direct sunlight. Recommended storage temperature is 4ºC.

Directions for Use

(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20. 
 (b) Add 100 μl of TMB solution/well. Incubate for specific amount of time or until desired signal is achieved. See product datasheet for recommended incubation times for specific antibody pair. 
 (c) Add 100 μl of STOP solution. 
 (d) Read Absorbance at 450 nm.

Background

TMB (3,3', 5,5"-tetramethylbenzidine) is used as a substrate to generate detectable signal in ELISA. The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measureable color change that correlates with analyte level.

注意事项：

Storage: Store at 4°C.