RAGE 1 (D1A12) Rabbit mAb

• 产品编号：CST-6996S      品牌：CST       原厂货号：6996S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RAGE 1 (D1A12) Rabbit mAb兔单抗能够检测内源水平RAGE1蛋白总体水平。此单克隆抗体是通过合成人源对应的RAGE 1 C-末端周围的肽段来免疫动物而获得。糖基化末端的终产物受体(RAGE)是免疫血球素(Ig)超家族的成员之一。它能够表达成全长细胞膜结合的RAGE 1型蛋白或者是作为缺少跨膜域的分泌型蛋白(1)。RAGE在发育的早期和肺在正常的生理条件下可以检测到(2)并且也可以在炎症位点高表达(3)。糖基化终末产物(AGEs)受体和另外很多的配体相互作用(1)。配体的结合激活全长的RAGE并起始下游的信号通路包括激活 NF-κB，这就导致了促炎症因子和炎症的产生(4)。 这些信号通路的激活与很多的疾病状态有关包括阿尔茨海默病, 肥胖症, 关节炎与动脉粥样硬化(4)。在细胞外环境，可溶性RAGE可竞争性结合RAGE配体，防止配体与全长RAGE在细胞表面的相互作用(1)。

1. Bierhaus, A. et al. (2005) J Mol Med 83, 876-86.
2. Brett, J. et al. (1993) Am J Pathol 143, 1699-712.
3. Sparvero, L.J. et al. (2009) J Transl Med 7, 17.
4. Lin, L. et al. (2009) Front Biosci 14, 1403-13.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

RAGE 1 (D1A12) Rabbit mAb recognizes endogenous levels of total RAGE 1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RAGE 1 protein.

Background

The receptor for advanced glycation end products (RAGE) is member of the immunoglobulin (Ig) superfamily. It can be expressed as full-length, membrane-bound RAGE isoform 1 or as a secreted sRAGE protein that lacks a transmembrane domain (1). RAGE is detected during early developmental stages and in the lung under normal physiological conditions (2) and is upregulated at sites of inflammation (3). Advanced glycation end products (AGEs) and a variety of other ligands interact with this receptor (1). Ligand binding activates full-length RAGE and initiates downstream signaling pathways that include activation of NF-κB, which leads to production of pro-inflammatory cytokines and inflammation (4). Activation of these pathways has been implicated in various disease states including Alzheimer disease, diabetes, arthritis, and atherosclerosis (4). Soluble RAGE can competitively bind RAGE ligands in the extracellular environment, which prevents ligand interaction with full-length RAGE at the cell surface (1).

1. Bierhaus, A. et al. (2005) J Mol Med 83, 876-86.
2. Brett, J. et al. (1993) Am J Pathol 143, 1699-712.
3. Sparvero, L.J. et al. (2009) J Transl Med 7, 17.
4. Lin, L. et al. (2009) Front Biosci 14, 1403-13.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.