免疫电泳后出现一条针对抗羊血清、兔IgG和兔血清的沉淀弧，没有出现针对抗胃蛋白酶、抗羊IgGFc或人或小鼠血清蛋白的反应。该试剂是用兔源IgG全分子免疫羊生产的， Anti-Rabbit IgG F(ab’)2 Fragment 是通过采用兔源IgG偶联琼脂糖微球的免疫亲和色谱法中的单一抗血清制备的，然后采用固相吸附去除不必要的反应如胃蛋白酶消化和色谱分离。此种抗体经初级氨基酸团与N-基琥珀酰亚胺酯活化的琼脂糖微球共价结合固定。 Anti-Rabbit IgG F(ab’)2 Fragment (结合琼脂糖微球) 用于兔源抗体的免疫沉淀。该试剂适用于免疫沉淀。其二抗可以检测兔源抗体。用F(ab’)2片段制作的二抗，可以减少细胞表面Fc受体的非特异性结合。
原厂资料：
Specificity / Sensitivity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgG and Rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human or mouse serum proteins.
Source / Purification
Anti-rabbit IgG F(ab')2 Fragment is produced by immunizing goats with rabbit IgG whole molecules. The Anti-rabbit IgG F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.
Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-rabbit IgG F(ab') 2 Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in rabbit.
Directions for Use
Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.
Background
Anti-rabbit IgG F(ab')2 Fragment (Sepharose Bead Conjugate) is ideal for immunoprecipitation. This secondary antibody will detect rabbit antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.
注意事项：
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not
aliquot the antibody.
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