Mouse His6Interleukin-33 (mHis6IL-33)

• 产品编号：CST-6983LC      品牌：CST       原厂货号：6983LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司利用重组的鼠_His6IL-33 (m_His6IL-33) Ser109-Ile266 (Accession #NP_598536)在E.coli细胞中表达。重组的mIL-33蛋白在N-末端有His6标签，其分子量据推算为19,439Da。DTT-还原和未还原的蛋白迁移时是作为一个22kDa 的多肽而转移。6 μg 还原 (+) 和 非还原的(-) m_His6IL-33通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白m_His6IL-33的生物活性是通过对MC/9细胞诱导产生IL-13的实验确定的。每个批次的ED50在1-6 ng/ml之间。内毒素含量:<0.01 ng /1 μg m_His6IL-33。

有载体: 每微克m_His6IL-33蛋白溶解在包含1 mM EDTA、3 mM DTT和 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克m_His6IL-33蛋白溶解在包含1 mM EDTA 和 3 mM DTT的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-33是IL-1蛋白家族中新的成员，此蛋白是在利用计算机技术鉴定未知家族成员时发现的(1)。IL-33 在纤维原和上皮细胞中表达, 并在炎症刺激中上调表达(1)。IL-33在Th2类型的炎症应答中发挥了重要的作用, 包括遗传性过敏症反应和哮喘(1,2)。IL-33受体由ST2 和 IL-1附属蛋白组成(IL-1-Acp) (1,3)。ST2在柱状细胞、嗜碱细胞和Th2细胞上表达(1)。除了作为Th2细胞因子的潜在功能外，IL-33也具有细胞核定位域并有可能具有细胞核因子的功能(4,5)。IL-33的细胞核功能依然不清楚。IL-33与其它的“报警因子”相似，通过细胞坏死而释放,从而向周围受伤组织发出信号(5,6)。然而，近来越来越多的数据表明IL-33也能通过非经典机制从活细胞中有活性的分泌出来(7)。

原厂资料：

Source / Purification

Recombinant mouse His6IL-33 (mHis6IL-33) Ser109-Ile266 (Accession #NP_598536) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged mIL-33 has a calculated MW of 19,439. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6IL-33. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mHis6IL-33 was determined by its ability to induce IL-13 production by MC/9 cells. The ED50 of each lot is between 1-6 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mHis6IL-33.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA, 3 mM DTT and 20 μg BSA per 1 μg mHis6IL-33. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM EDTA and 3 mM DTT.

Background

IL-33 is a novel member of the IL-1 family of proteins that was discovered in a computational search to identify unknown family members (1). IL-33 is expressed by fibroblasts and endothelial cells, and is upregulated in response to inflammatory stimuli (1). IL-33 plays a key role in Th2 type inflammatory responses, including atopic allergic responses and asthma (1,2). The IL-33 receptor consists of the ST2 and IL-1 accessory protein (IL-1-Acp) (1,3). ST2 is expressed on mast cells, basophils and Th2 cells (1). In addition to its putative role as a Th2 cytokine, IL-33 also contains a nuclear localization domain and may function as a nuclear factor (4,5). The nuclear function of IL-33 remains unclear. IL-33 is released by cellular necrosis, similar to other “alarmins”, thereby signaling tissue injury (5,6). However, more recent data suggests that IL-33 may also be actively secreted from live cells via a non-classical mechanism (7).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.