PAR2 (D61D5) Rabbit mAb

• 产品编号：CST-6976S      品牌：CST       原厂货号：6976S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PAR2 (D61D5) Rabbit mAb兔单抗识别内源性PAR2总蛋白。主要条带在32 kDa大小被检测，更大的分子量被检测是由于糖基化。通过人工合成人源PAK2蛋白Gly44位点相应的多肽片段去免疫动物从而制备出单克隆抗体。PAR2 (F2RL1)蛋白属于G蛋白偶联受体的PAR(Protease-activated Receptor)家族。通过一个丝氨酸蛋白酶例如凝血酶、胰岛素或基质金属蛋白酶剪切该受体的N端从而使这些膜受体激活(1,2)。这种剪切展示该受体系锁配体(tethered ligand)片段，这结合到该受体的第二个细胞外环，并且导致受体的激活。PAR2蛋白通过胰蛋白酶或胰岛素样蛋白酶被特异性激活。活化的PAR2蛋白激活磷酸肌醇水解作用和钙的调动，并且能与 β-arrestin相互作用以及最终导致ERK的激活(3)。PAR2蛋白表达和激活主要与炎症疾病相关 (3)，但是也可能在癌症的发展中起到重要作用(4,5)。

1. Macfarlane, S.R. et al. (2001) Pharmacol Rev 53, 245-82.
2. Ossovskaya, V.S. and Bunnett, N.W. (2004) Physiol Rev 84, 579-621.
3. Bushell, T. (2007) J Physiol 581, 7-16.
4. Su, S. et al. (2009) Oncogene 28, 3047-57.
5. Gessler, F. et al. (2010) Neuroscience 165, 1312-22.

原厂资料：

Specificity / Sensitivity

PAR2 (D61D5) Rabbit mAb recognizes endogenous levels of total PAR2 protein. The major band detected runs at 32 kDa, the higher molecular weight smear is due to glycosylation.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly44 of human PAR2 protein.

Background

PAR2 (F2RL1) belongs to the PAR (Protease-activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through N-terminal cleavage of the receptor by a serine protease such as thrombin, trypsin, or matrix metalloproteinases (1,2). This cleavage exposes the ‘tethered-ligand’ fragment of the receptor, which binds to a second extracellular loop of the receptor and leads to receptor activation. PAR2 is specifically activated by trypsin or trypsin-like proteases. Activated PAR2 stimulates phosphoinositide hydrolysis and calcium mobilization, interacts with β-arrestin, and eventually leads to ERK activation (3). PAR2 expression and activation are mainly associated with inflammatory diseases (3), but may also play a role in cancer development (4,5).

1. Macfarlane, S.R. et al. (2001) Pharmacol Rev 53, 245-82.
2. Ossovskaya, V.S. and Bunnett, N.W. (2004) Physiol Rev 84, 579-621.
3. Bushell, T. (2007) J Physiol 581, 7-16.
4. Su, S. et al. (2009) Oncogene 28, 3047-57.
5. Gessler, F. et al. (2010) Neuroscience 165, 1312-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody