Rab8A (D22D8) XP? Rabbit mAb

• 产品编号：CST-6975S      品牌：CST       原厂货号：6975S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab8 (D22D8) XP® Rabbit mAb兔单抗识别内源性的Rab8总蛋白水平。该单克隆抗体用与人类Rab8蛋白中Lys175位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。Rab8 GTP酶是Ras超家族的成员，与蛋白转运和膜重构有关（1）。研究显示Rab8定位于高尔基网络反面（TGN）、基底外侧膜和囊泡结构，帮助调节目标蛋白在TGN和基底外侧膜之间的转运（1-3）。对Rab8和结合的Rab8GEF的过表达研究和突变分析表明其还在极化膜运输和膜融合形成中对肌动蛋白和微管蛋白的重塑发挥作用（4-6）。Rab8与肌球蛋白Vb结合，为肌肉接受胰岛素刺激后的GLUT4转位所需（7,8）。Rab8对目标蛋白囊泡转运的控制也能调节细胞外基质形成时MT1-MMP活性以及在紧密连接处形成过程中时的JRAB/MICAL-L2（9,10）。

1. Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12.
2. Huber, L.A. et al. (1993) J Cell Biol 123, 35-45.
3. Henry, L. and Sheff, D.R. (2008) Mol Biol Cell 19, 2059-68.
4. Peränen, J. et al. (1996) J Cell Biol 135, 153-67.
5. Hattula, K. et al. (2002) Mol Biol Cell 13, 3268-80.
6. Hattula, K. et al. (2006) J Cell Sci 119, 4866-77.
7. Ishikura, S. and Klip, A. (2008) Am J Physiol Cell Physiol 295, C1016-25.
8. Randhawa, V.K. et al. (2008) J Biol Chem 283, 27208-19.
9. Bravo-Cordero, J.J. et al. (2007) EMBO J 26, 1499-510.
10. Yamamura, R. et al. (2008) Mol Biol Cell 19, 971-83.

原厂资料：

Specificity / Sensitivity

Rab8 (D22D8) XP® Rabbit mAb recognizes endogenous levels of total Rab8 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys175 of human Rab8 protein.

Background

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring (1). Studies show that Rab8 is localized to the trans Golgi network (TGN), basolateral membrane, and vesicular structures where it helps regulate target protein transport between TGN and the basolateral membrane (1-3). Overexpression studies and mutation analysis of Rab8 and its associated Rab8GEF indicate additional roles in actin and microtubule remodeling during polarized membrane transport and membrane protrusion formation (4-6). Rab8 associates with myosin Vb and is required for translocation of GLUT4 following insulin stimulation in muscle (7,8). Control of target protein vesicle transport by Rab8 also regulates MT1-MMP activity during extracellular matrix formation and JRAB/MICAL-L2 at tight junction formation (9,10).

1. Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12.
2. Huber, L.A. et al. (1993) J Cell Biol 123, 35-45.
3. Henry, L. and Sheff, D.R. (2008) Mol Biol Cell 19, 2059-68.
4. Peränen, J. et al. (1996) J Cell Biol 135, 153-67.
5. Hattula, K. et al. (2002) Mol Biol Cell 13, 3268-80.
6. Hattula, K. et al. (2006) J Cell Sci 119, 4866-77.
7. Ishikura, S. and Klip, A. (2008) Am J Physiol Cell Physiol 295, C1016-25.
8. Randhawa, V.K. et al. (2008) J Biol Chem 283, 27208-19.
9. Bravo-Cordero, J.J. et al. (2007) EMBO J 26, 1499-510.
10. Yamamura, R. et al. (2008) Mol Biol Cell 19, 971-83.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.