Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab? Rabbit mAb mix

• 产品编号：CST-6967S      品牌：CST       原厂货号：6967S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb可识别内源性的+2和-2位是精氨酸或赖氨酸的磷酸化丝氨酸。此抗体可能与包含S*X(R/K)基序的蛋白有交叉反应。该抗体不与非磷酸化丝氨酸残基、同样基序中磷酸化的苏氨酸残基、其它基序中的磷酸化丝氨酸残基发生交叉反应。该单克隆抗体用合成的磷酸化丝氨酸PKC底物免疫动物制备。该抗体是两个兔单克隆的混合，以便可以覆盖更大的反应范围。尽管蛋白激酶C（PKC）家族成员参与多种信号转导过程，包括分泌、基因表达、增殖和肌肉收缩（1,2），但它们的很多底物仍然未确定。PKC的同工酶分为传统型（cPKC），新型PKC（nPKC）和非典型PKC（aPKC）。PKCα, βI, βII, 和γ属于cPKC组（1）。激活后cPKC同工酶磷酸化精氨酸或赖氨酸在-3,-2和+2位，和+1位是疏水氨基酸的丝氨酸和苏氨酸（1-3）。为了鉴别潜在的PKC底物，Cell Signaling Technology公司开发了识别此基序的抗体。Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb兔单抗特异性识别包含磷酸化丝氨酸的cPKC底物。

1. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
3. Obata, T. et al. (2000) J Biol Chem 275, 36108-15.

原厂资料：

Specificity / Sensitivity

Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb detects endogenous levels of many cellular proteins only when phosphorylated at Ser residues surrounded by Arg or Lys at the -2 and +2 positions. The antibody may also cross-react with S*X(R/K) motif-containing proteins. The antibody does not cross-react with nonphosphorylated Ser residues, phospho-Thr in the same motif, or phospho-Ser in other motifs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-(Ser) PKC substrate peptides. The antibody is formulated from three rabbit monoclones in order to cover a broad range of reactivity.

Background

Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser.

1. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
3. Obata, T. et al. (2000) J Biol Chem 275, 36108-15.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.