CNOT2 Antibody

• 产品编号：CST-6955S      品牌：Cell Signaling Technology       原厂货号：6955S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CNOT2 Antibody能够检测内源性的 CNOT2总蛋白水平。该多克隆抗体是经由合成的人CNOT2 蛋白近羧基端的氨基酸肽段免疫动物而生产的。经过Protein A和亲和层析纯化。进化保守的CCR4-NOT (CNOT) 复合物能够调控真核细胞中的mRNA代谢（1）。这种调控作用发生在mRNA合成和降解的不同水平，包括转录起始，延伸，去乙酰化以及降解（1）。已经鉴定出了该复合物中存在的多种组分包括CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT7, CNOT8, CNOT9, 以及CNOT10（2）。另外，该复合物的亚基组成在不同的组织中是各异的（3）。

1. Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
2. Lau, N.C. et al. (2009) Biochem J 422, 443-53.
3. Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.

原厂资料：

Specificity / Sensitivity

CNOT2 Antibody recognizes endogenous levels of total CNOT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CNOT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3).

1. Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
2. Lau, N.C. et al. (2009) Biochem J 422, 443-53.
3. Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.