TRXR1 Antibody

• 产品编号：CST-6925S      品牌：CST       原厂货号：6925S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TRXR1 Antibody用于检测内源性TRXR1总蛋白水平。该多抗通过对应于人源TRXR1蛋白Ile259位点周围残基序列的合成肽免疫动物制备而得。抗体经Protein A和肽亲和层析纯化。硫氧还蛋白还原酶1（TRXR1)是一种含硒代半胱氨酸的蛋白，主要在氧化还原稳态中发挥作用（1－6）。它的底物是硫氧还蛋白，是另外一种氧化还原蛋白（1）。它们共同发挥着许多广泛的功能，如抗氧化调节反应（3－6），细胞增殖（2,3，5），DNA的复制（2,3）和转录过程（3,5）。TRXR1能够还原多种细胞蛋白（1,3）。硒的缺乏，不管是由于饮食原因还是由于甲基水银的引入，都会影响TRXR1的表达和功能。很有可能正是这种效应导致了高氧化状态，而这种效应是由于缺少足够量的硒元素，导致硒半胱氨酸密码子UGA被错误地读作了终止子（4）。TRXR1的细胞增殖和抗氧化效应使得它可以作用药物的一个潜在靶点。

1. Turanov, A.A. et al. (2010) Biochem J 430, 285-93.
2. Gasdaska, P.Y. et al. (1995) FEBS Lett 373, 5-9.
3. Gromer, S. et al. (2004) Med Res Rev 24, 40-89.
4. Usuki, F. et al. (2011) J Biol Chem 286, 6641-9.
5. Pappas, A.C. et al. (2008) Comp Biochem Physiol B Biochem Mol Biol 151, 361-72.
6. Müller, M. et al. (2010) Genes Nutr 5, 297-307.

原厂资料：

Specificity / Sensitivity

TRXR1 Antibody recognizes endogenous levels of total TRXR1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile259 of human TRXR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TRXR1 (thioredoxin reductase 1) is a selenocysteine-containing protein that is involved in redox homeostasis (1-6). Its canonical target is thioredoxin, another redox protein (1). Together, they are involved in many functions such as antioxidant regulation (3-6), cell proliferation (2,3,5), DNA replication (2,3), and transcription (3,5). TRXR1 is also capable of reducing a wide array of cellular proteins (1,3). Selenium deficiency, either by diet modification (2,6) or introduction of methylmercury (4), hinders proper expression and function of TRXR1. It is possible that this effect, which results in a higher oxidative state, is a result of the selenocysteine codon (UGA) being read as a STOP codon in the absence of adequate selenium (4). The functions of TRXR1 in cell proliferation and antioxidant defense make it a potential therapeutic target.

1. Turanov, A.A. et al. (2010) Biochem J 430, 285-93.
2. Gasdaska, P.Y. et al. (1995) FEBS Lett 373, 5-9.
3. Gromer, S. et al. (2004) Med Res Rev 24, 40-89.
4. Usuki, F. et al. (2011) J Biol Chem 286, 6641-9.
5. Pappas, A.C. et al. (2008) Comp Biochem Physiol B Biochem Mol Biol 151, 361-72.
6. Müller, M. et al. (2010) Genes Nutr 5, 297-307.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody