IRAP (D7C5) XP? Rabbit mAb

• 产品编号：CST-6918S      品牌：CST       原厂货号：6918S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRAP (D7C5) XP® Rabbit mAb兔单抗识别内源性IRAP总蛋白水平。单抗通过用与人IRAP氨基端序列相同的融合蛋白免疫动物而得。IRAP(也称作LNPEP)最初认为是一种位于含Glut4囊泡上的胰岛素反应性氨肽酶(1)。它不仅与Glut4有严格的共定位，而且由胰岛素刺激的蛋白转位模式也与Glut4相同(2)。因此，普遍认为IRAP是Glut4的一个很好的标志(2)。后来发现IRAP是一种调控着一些激素和调节蛋白表达与活性的关键酶(3,4)。作用一种锌依赖型的半胱氨酸氨肽酶膜蛋白，IRAP不仅是血管紧张素的受体，同时它也调控着血管紧张素原前体生成血管紧张素的生化途径(5)。IRAP在一些肽酶的水解过程中起催化作用，这其中包括催产素和加压素等(4)。IRAP的异常表达跟人体的几种癌症相关，包括肾脏癌症和子宫内膜癌(6,7)。

1. Garza, L.A. and Birnbaum, M.J. (2000) J Biol Chem 275, 2560-7.
2. Gross, D.N. et al. (2004) Mol Cell Biol 24, 7151-62.
3. Albiston, A.L. et al. (2001) J Biol Chem 276, 48623-6.
4. Keller, S.R. (2003) Front Biosci 8, s410-20.
5. Vanderheyden, P.M. (2009) Mol Cell Endocrinol 302, 159-66.
6. Larrinaga, G. et al. (2007) Regul Pept 144, 56-61.
7. Suzuki, Y. et al. (2003) Clin Cancer Res 9, 1528-34.

原厂资料：

Specificity / Sensitivity

IRAP (D7C5) XP® Rabbit mAb recognizes endogenous levels of total IRAP protein.

Source / Purification

Monoclonal antibody is produced by immunizing ani

Background

IRAP (also known as LNPEP) was originally described as an insulin-responsive aminopeptidase found in Glut4-containing vesicles (1). It is essentially always in the same compartments as Glut4 and has identical insulin-stimulated translocation patterns as Glut4 (2). IRAP is therefore considered to be a surrogate marker for Glut4 (2). IRAP was later found to be a critical enzyme that regulates the expression and activity of several essential hormones and regulatory proteins, including the Glut4 transporter (3,4). This membrane associated, zinc-dependent cystinyl aminopeptidase acts as both a receptor for angiotensin IV as well as the enzyme that catalyzes the synthesis of this essential hormone from its angiotensinogen precursor (5). IRAP catalyzes the hydrolysis of several peptide hormones, including oxytocin and vasopressin (4). Abnormal IRAP expression or activity is associated with several forms of cancer in humans, including renal and endometrial cancers (6,7).

1. Garza, L.A. and Birnbaum, M.J. (2000) J Biol Chem 275, 2560-7.
2. Gross, D.N. et al. (2004) Mol Cell Biol 24, 7151-62.
3. Albiston, A.L. et al. (2001) J Biol Chem 276, 48623-6.
4. Keller, S.R. (2003) Front Biosci 8, s410-20.
5. Vanderheyden, P.M. (2009) Mol Cell Endocrinol 302, 159-66.
6. Larrinaga, G. et al. (2007) Regul Pept 144, 56-61.
7. Suzuki, Y. et al. (2003) Clin Cancer Res 9, 1528-34.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.