Miwi (D92B7) XP? Rabbit mAb

• 产品编号：CST-6915P      品牌：Cell Signaling Technology       原厂货号：6915P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Miwi (D92B7) XP® Rabbit mAb兔单抗检测内源性Miwi总蛋白。通过人工合成小鼠Miwi蛋白Gly81位点周围序列相应多肽片段去免疫动物从而制备单克隆抗体。果蝇piwi 基因被鉴定是生殖系干细胞自我更新所必须的(1)。在不同的物种包括拟南芥、秀丽隐杆线虫和人类中，Piwi同源物都很保守(1)。Miwi和Mili蛋白都是小鼠Piwi 同源物，且都包含有 C末端Piwi结构域(2)。Miwi和Mili蛋白结合到在雄性生殖细胞中Piwi-interacting RNAs (piRNAs)，并且对小鼠精子的生成是至关重要的(3-5)。

1. Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
2. Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
3. Aravin, A. et al. (2006) Nature 442, 203-7.
4. Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
5. Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Rat

Specificity / Sensitivity

Miwi (D92B7) XP® Rabbit mAb recognizes endogenous levels of total Miwi protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly81 of mouse Miwi protein.

Background

The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).

1. Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
2. Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
3. Aravin, A. et al. (2006) Nature 442, 203-7.
4. Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
5. Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody