SignalSilence? PI3 Kinase p85α siRNA I (Mouse Specific)

• 产品编号：CST-6912S      品牌：CST       原厂货号：6912S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® PI3 Kinase p85α siRNA I (Mouse Specific) 可以帮助研究者通过RNA干扰特异性地抑制PI3 Kinase p85α的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® PI3 Kinase p85α siRNA I (Mouse Specific）进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。PI3K激酶通过磷酸化磷脂酰肌醇(PI), 4-磷酸磷脂酰肌醇 (PIP)和4，5-二磷酸磷脂酰肌醇 (PIP2)催化3,4,5-三磷酸磷脂酰肌醇的产生。生长因子和激素会引发该磷酸化进程，随后协助细胞生长，细胞周期进入，细胞迁移和细胞存活（1）。PTEN会逆转该过程，研究表明PI3K信号通路在PTEN功能缺失的人类癌症中是持续性激活的（2）。PI3Ks由一个催化亚基（p110）和一个调控亚基构成。已经分离得到了多个催化亚基(p110α, p110β, p110γ, 和p110δ)，与p110α, p110β, 和 p110δ结合的调控亚基是p85α 和 p85β (3).相对的，与p101调控亚基结合的p110γ与p85无关。此外，异源三聚体G蛋白的βγ亚基可以激活p110γ(4)。

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
4. Stoyanov, B. et al. (1995) Science 269, 690-3.

原厂资料：

Description

SignalSilence® PI3 Kinase p85α siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PI3 Kinase p85α expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® PI3 Kinase p85-α siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
4. Stoyanov, B. et al. (1995) Science 269, 690-3.

注意事项：

Storage: PI3 Kinase p85α siRNA I (Mouse Specific) is supplied
in RNAse-free water. Aliquot and store at -20ºC.