FAIM Antibody

• 产品编号：CST-6907S      品牌：CST       原厂货号：6907S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FAIM Antibody能够检测内源性水平FAIM总蛋白。该多克隆抗体是采用合成的人源FAIM蛋白Gly145周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。FAIM（Fas凋亡抑制子）是在B淋巴细胞中被诱导表达的，是一种能够抗Fas介导的细胞凋亡的蛋白质（1）。FAIM的表达能够抑制B细胞以及其他类型细胞中受体介导的细胞凋亡（1-3）。FAIM在胚芽中心的B细胞中表达，受IRF-4的正调控，也能够通过前反馈机制诱导IRF-4的表达（4）。FAIM还通过调节Akt的活化与Nur77的表达来调节T细胞受体介导的凋亡（2）。 通过对FAIM基因敲除的小鼠的研究表明，在B细胞、T细胞和肝细胞内，Fas介导的细胞凋亡的敏感性增加（5）。科学家主要在大脑中发现 FAIM有一种称为FAI M-L的选择性剪接（6）。在神经系统中，最初认为FAIM不会在细胞凋亡中发挥作用，而是通过ERK的活化和NF-κB途径促进神经轴突生长（7）。相反的，FAIM-L会通过死亡受体抑制神经细胞死亡的发生（3）。

1. Schneider, T.J. et al. (1999) J Exp Med 189, 949-56.
2. Huo, J. et al. (2010) J Biol Chem 285, 11827-35.
3. Segura, M.F. et al. (2007) J Neurosci 27, 11228-41.
4. Kaku, H. and Rothstein, T.L. (2009) J Immunol 183, 5575-81.
5. Huo, J. et al. (2009) Cell Death Differ 16, 1062-70.
6. Zhong, X. et al. (2001) Mol Immunol 38, 65-72.
7. Sole, C. et al. (2004) J Cell Biol 167, 479-92.

原厂资料：

Specificity / Sensitivity

FAIM Antibody recognizes endogenous levels of total FAIM protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly145 of human FAIM protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

FAIM (Fas apoptosis inhibitory molecule) was identified as a protein that was inducibly expressed in B lymphocytes resistant to Fas-mediated apoptosis (1). Expression of FAIM inhibits receptor-mediated apoptosis in B cells as well as other cell types (1-3). FAIM is expressed in germinal center B cells, is positively regulated by IRF-4, and is also capable of inducing IRF-4 expression in a feed-forward mechanism (4). FAIM also regulates T cell receptor-mediated apoptosis by modulating Akt activation and Nur77 expression (2). Knockout mice for FAIM show an increased sensitivity to Fas-mediated apoptosis within B and T cells as well as hepatocytes (5). An alternatively spliced form of FAIM, termed FAIM-L, is found predominantly in the brain (6). In the nervous system, the originally identified FAIM does not appear to play a role in apoptosis, but rather can promote neurite outgrowth through the activation of Erk and NF-κB pathways (7). In contrast, FAIM-L does inhibit neuronal cell death triggered by death receptors (3).

1. Schneider, T.J. et al. (1999) J Exp Med 189, 949-56.
2. Huo, J. et al. (2010) J Biol Chem 285, 11827-35.
3. Segura, M.F. et al. (2007) J Neurosci 27, 11228-41.
4. Kaku, H. and Rothstein, T.L. (2009) J Immunol 183, 5575-81.
5. Huo, J. et al. (2009) Cell Death Differ 16, 1062-70.
6. Zhong, X. et al. (2001) Mol Immunol 38, 65-72.
7. Sole, C. et al. (2004) J Cell Biol 167, 479-92.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.