CHFR (D40H6) Rabbit mAb

• 产品编号：CST-6904S      品牌：CST       原厂货号：6904S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CHFR (D40H6) Rabbit mAb能够检测内源性CHFR总蛋白。该单克隆抗体是由合成的人源的针对CHFR 蛋白赖氨酸（612位）的肽段免疫动物生产的。细胞有丝分裂前期检查点蛋白(CHFR)是一种E3泛素蛋白连接酶，它能够调节细胞周期进程。在微管应激反应的作用下，CHFR先于染色体凝聚排除核中的周期蛋白B1从而延迟细胞进入有丝分裂(1)。在一些原发性肿瘤中已检测到CHFR表达的显著减少，而其表达的降低和启动子甲基化有关(1-4)。有研究报道采用微管应激反应试剂诺考达唑和甲基转移酶抑制剂5-叠氮-脱氧胞嘧啶处理能够回复CHFR的表达(4,5)。

1. Summers, M.K. et al. (2005) Oncogene 24, 2589-98.
2. Privette, L.M. et al. (2007) Cancer Res 67, 6064-74.
3. Morioka, Y. et al. (2006) Anticancer Res 26, 4267-70.
4. Satoh, A. et al. (2003) Cancer Res 63, 8606-13.
5. Dang, C. and Hudis, C. (2006) Clin Breast Cancer 7, 51-8.

原厂资料：

Specificity / Sensitivity

CHFR (D40H6) Rabbit mAb recognizes endogenous levels of total CHFR protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys612 of human CHFR protein. 

Background

Checkpoint with forkhead and RING finger domains protein (CHFR) is an E3 ubiquitin-protein ligase that regulates cell cycle progression. In response to microtubule stress, CHFR delays the transition into mitosis by excluding cyclin B1 from the nucleus prior to chromosome condensation (1). Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation (1-4). Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported (4,5). 

1. Summers, M.K. et al. (2005) Oncogene 24, 2589-98.
2. Privette, L.M. et al. (2007) Cancer Res 67, 6064-74.
3. Morioka, Y. et al. (2006) Anticancer Res 26, 4267-70.
4. Satoh, A. et al. (2003) Cancer Res 63, 8606-13.
5. Dang, C. and Hudis, C. (2006) Clin Breast Cancer 7, 51-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody