SMC1 (8E6) Mouse mAb

• 产品编号：CST-6892S      品牌：CST       原厂货号：6892S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SMC1 (8E6) Mouse mAb能够识别内源性SMC1总蛋白的水平。该单克隆抗体是由针对人SMC1氨基末端的特异性重组蛋白免疫动物生产的。黏连蛋白复合物是由介于SMC1 (SMC1A or B)和SMC3之间的异源二聚体组成，邻接RAD21和STAG蛋白(STAG1，2或3) (1,2)。这些蛋白形成一个环状结构来介导S期DNA复制后姐妹染色单体的结合(1,2)。RAD21和STAGE2可在分裂前期被Polo样激酶(PLK)磷酸化，导致黏连蛋白复合体从染色体臂分离；然而，黏连蛋白仍然结合中中心体直至分裂后期(3,4)。RAD21可以在分裂后期被分离蛋白/ESPL1裂解，导致剩下的黏连蛋白从中心体分离，促进有丝分裂中姐妹染色单体的分离(5)。RAD21也能够在凋亡中被凋亡蛋白酶-3和凋亡蛋白酶-7裂解，导致一个64kDa的羧基末端裂解产物向胞质转移并有助于触发凋亡(6,7)。除了介导姐妹染色单体的结合，黏连蛋白复合物在基因调控和DNA修复中发挥重要作用，因为在DNA损伤处SMC1和SMC3都被ATM和ATR激酶磷酸化(1,2)。

1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114.
2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67.
4. Hauf, S. et al. (2005) PLoS Biol 3, e69.
5. Hauf, S. et al. (2001) Science 293, 1320-3.
6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77.
7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.

原厂资料：

Specificity / Sensitivity

SMC1 (8E6) Mouse mAb recognizes endogenous levels of total SMC1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SMC1 protein. 

Background

The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). 

1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114.
2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67.
4. Hauf, S. et al. (2005) PLoS Biol 3, e69.
5. Hauf, S. et al. (2001) Science 293, 1320-3.
6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77.
7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.