RBAP46 (V415) Antibody

• 产品编号：CST-6882S      品牌：CST       原厂货号：6882S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RBAP46 (V415) Antibody能够检测内源性RBAP46总蛋白水平。该抗体不能与RBAP48蛋白交叉反应。通过合成的与人源RBAP46蛋白羧基端相应多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Retinoblastoma-associated proteins 46和48 (RBAP46和RBAP48;又称为RBBP7和RBBP4)在人源细胞中首先以结合到retinoblastoma (Rb)肿瘤抑制蛋白为特征(1)。从那时以来，这些蛋白质已经显示是许多蛋白质复合物中的成分，这些复合物涉及染色质调节，包括chromatin assembly factor 1 (CAF-1)复合物和type B组蛋白乙酰转移酶复合物HAT1，在DNA复制期间这两个复合物的功能是染色质的装配(2,3)。RBAP46和RBAP48蛋白也被发现在核小体重构因子复合物NURF、核小体重塑和组蛋白去乙酰化复合物NuRD和Sin3/HDAC组蛋白去乙酰化复合物(4-7)。最近，RBAB46和RBAP48被鉴定作为polycomb repressor complex PRC2的成分，该复合物也包含EED和Ezh2 (8)。RBAP46和RBAP48结合到组蛋白histone H4折叠区域，以及认为有助于这些染色质重塑、组蛋白乙酰化和组蛋白去乙酰化复合物作用于它们的组蛋白底物上(3)。

1. Qian, Y.W. et al. (1993) Nature 364, 648-52.
2. Verreault, A. et al. (1996) Cell 87, 95-104.
3. Verreault, A. et al. (1998) Curr Biol 8, 96-108.
4. Barak, O. et al. (2003) EMBO J 22, 6089-100.
5. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
6. Zhang, Y. et al. (1997) Cell 89, 357-64.
7. Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905.
8. Cao, R. et al. (2002) Science 298, 1039-43.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig, Horse

Specificity / Sensitivity

RBAP46 (V415) Antibody recognizes endogenous levels of total RBAP46 protein. This antibody does not cross-react with the RBAP48 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RBAP46 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAB46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3).

1. Qian, Y.W. et al. (1993) Nature 364, 648-52.
2. Verreault, A. et al. (1996) Cell 87, 95-104.
3. Verreault, A. et al. (1998) Curr Biol 8, 96-108.
4. Barak, O. et al. (2003) EMBO J 22, 6089-100.
5. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
6. Zhang, Y. et al. (1997) Cell 89, 357-64.
7. Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905.
8. Cao, R. et al. (2002) Science 298, 1039-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.