SignalSilence? ABIN-1 siRNA II

• 产品编号：CST-6609S      品牌：CST       原厂货号：6609S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

CST生产的SignalSilence® ABIN-1 siRNA II可以特异性的扰特ABIN-1的表达，此方法是通过传递双链RNA到细胞中从而选择性的沉默目的基因。所有的SignalSilence® siRNA 产品经过严格的公司内部测试并通过Western验证可有效的降低蛋白的表达。寡聚核苷酸的合成是在三苯甲游基的控制下保持适当的结合效率。寡聚链随后通过亲和固相提纯。退火的双链RNA通过质谱进一步的分析以确定抽提的成分。每一个批次都通过质谱跟前一个批次比较以确定批次间的连贯性。CST推荐转染条件为：转染100 nM ABIN-1 siRNA II，48-72小时后裂解细胞。至于转染的步骤需根据转染试剂的方法手册进行。如有问题请联系我们。ABIN 家族(ABIN-1, -2, and -3)是一组接头蛋白，与A20/TNFAIP3相关并协同作用(1), 它是一个编辑泛素的蛋白，并抑制主要的炎症转录因子NF-κB (2-4)。 理论上来讲 A20 通过调节激酶RIP的泛素化导致IKK复合体的抑制而起作用(5)。

1. Verstrepen, L. et al. (2009) Biochem Pharmacol 78, 105-14.
2. Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51.
3. Lee, E.G. et al. (2000) Science 289, 2350-4.
4. Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8.
5. Wertz, I.E. et al. (2004) Nature 430, 694-9.

原厂资料：

Description

SignalSilence® ABIN-1 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ABIN-1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM ABIN-1 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-κB (2-4). Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5).

1. Verstrepen, L. et al. (2009) Biochem Pharmacol 78, 105-14.
2. Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51.
3. Lee, E.G. et al. (2000) Science 289, 2350-4.
4. Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8.
5. Wertz, I.E. et al. (2004) Nature 430, 694-9.

注意事项：

Storage: ABIN-1 siRNA II is supplied in RNAse-free water.
Aliquot and store at -20ºC.