SignalSilence? Dicer siRNA II

• 产品编号：CST-6585S      品牌：CST       原厂货号：6585S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence® Dicer siRNA II能抑制人、小鼠和大鼠Dicer蛋白表达。Cell Signaling Technology (CST)公司的SignalSilence® Dicer siRNA II允许研究者通过RNA干扰去特异性抑制dicer蛋白表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。CST公司推荐在细胞裂解前48到72小时转染100 nM Dicer siRNA I。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Dicer是RNase III酶家族成员之一，它能特异性剪切双链RNAs从而产生microRNAs (miRNAs) (1)。在Drosha酶将长的初始转录本pri-miRNAs剪切成含有茎环pre-miRNAs之后(2)，pre-miRNAs被转运到细胞质，随后进一步被Dicer酶剪切成含22个核苷酸的成熟miRNAs(3)。然后，成熟miRNAs成为RNA诱导的沉默复合物（RNA-Induced Silencing Complex，RISC）的一部分，从而能够结合目标mRNA的3'端非编码区(3)。

1. Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60.
2. Lee, Y. et al. (2003) Nature 425, 415-9.
3. Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

SignalSilence® Dicer siRNA II will inhibit human, mouse and rat Dicer expression.

Description

SignalSilence® Dicer siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit dicer expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Directions for Use

CST recommends transfection with 100 nM Dicer siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3).

1. Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60.
2. Lee, Y. et al. (2003) Nature 425, 415-9.
3. Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108.

注意事项：

Storage: Dicer siRNA II is supplied in RNAse-free water. Aliquot
and store at -20°C.