RNA干扰技术是一种通过向细胞中输入双链RNA分子,选择性沉默基因表达的方法。一种特定的小干扰RNA的引入可以通过降解RNA来阻止目的蛋白的翻译。SignalSilence ® Control siRNA (未标记)是一种小干扰RNA(siRNA),不能导致细胞信号的特定降解,而用来作为靶siRNA转染的阴性对照。这种双链RNA与SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201具有相同的密码序列。建议裂解细胞前用100nM Control siRNA II 转染48-72个小时。请参考转染试剂生产商提供的转染步骤。如有问题,请联系CST公司。
原厂资料:
Description
RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. The introduction of a specific small interfering RNA (siRNA) strand prevents the translation of a target protein message through RNA degradation. SignalSilence® Control siRNA (Unconjugated) is a siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. This duplex contains the same scrambled sequence used in SignalSilence® Control siRNA (Fluorescein Conjugate) #6201.
Directions for Use
CST recommends transfection with 100 nM Control siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
注意事项:
Storage: SignalSilence ® Control siRNA (Unconjugated) is sup-
plied in RNAse-free water. Aliquot and store at -20ºC