SignalSilence? c-Myc siRNA II

• 产品编号：CST-6552S      品牌：CST       原厂货号：6552S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® c-Myc siRNA II可以帮助研究者通过RNA干扰特异性地抑制c-Myc的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® c-Myc siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Myc/Max/Mad网络成员作为转录调控因子,在细胞增殖、分化和凋亡等多种细胞行为方面发挥着重要功能（1）。这些蛋白质共享一个共同的基础螺旋-环-螺旋亮氨酸拉链(bHLH-ZIP)基序，这是二聚化和DNA结合所需的。Max最初是因其与c-Myc的互作能力而被发现，发现它对于Myc蛋白与DNA结合和激活转录过程是必须的（2）。随后，Max一直被视为转录网络的核心组成部分，与Myc和Mad家族其他成员形成同源二聚体和异源二聚体（1）。Max与Myc或Mad的互作对于转录调控和细胞行为可以有相反的作用（1）。Mad家族包括四个相关的蛋白质，Mad1、Mad2 (Mxi1)、Mad3和Mad4，以及bHLH-ZIP 家族最不相关的成员Mnt和Mga。与Myc一样，Mad蛋白严格被短半衰期调控。在一般情况下，Mad家族成员通过抑制转录干扰Myc蛋白介导的生命过程，如增殖、细胞凋亡的转化和阻止（3,4）。

1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.

原厂资料：

Description

SignalSilence^® c-Myc siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit c-Myc expression by RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM c-Myc siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).

1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.

注意事项：

Storage: c-Myc siRNA II is supplied in RNAse-free water.
Aliquot and store at -20°C.