SignalSilence? Tuberin/TSC2 siRNA II

• 产品编号：CST-6548S      品牌：CST       原厂货号：6548S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® Tuberin/TSC2 siRNA II 可以帮助研究者通过RNA干扰特异性地抑制tuberin/TSC2的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Tuberin/TSC2 siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。马铃薯球蛋白（Tuberin）是TSC2肿瘤抑制基因的产物，是细胞增殖和肿瘤发展的重要调节因子（1）。TSC2或与之相关的TSC1（hamartin）的突变可以引起结节硬化复合体TSC（tuberous sclerosis complex），是一种常染色体显性遗传病，其特点为发生多种广泛的非恶性肿瘤（2）。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化（3）。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性（3-5）。另外，tuberin可以抑制mTOR（mammalian target of rapamycin）， 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白（4E-BP1，一种转录起始抑制因子）的活性，并最终抑制翻译（3,6,7）。

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.

原厂资料：

Description

SignalSilence® Tuberin/TSC2 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit tuberin/TSC2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Tuberin/TSC2 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
3. Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
4. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
5. Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
6. Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
7. Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.

注意事项：

Storage: Tuberin/TSC2 siRNA II is supplied in RNAse-free
water. Aliquot and store at -20°C.