SignalSilence? Survivin siRNA II

• 产品编号：CST-6546S      品牌：CST       原厂货号：6546S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

Survivin siRNA II 能够抑制人和狗Survivin表达。来自Cell Signaling Technology (CST)的SignalSilence^® Survivin siRNA II可以帮助研究者通过RNA干扰特异性地抑制Survivin的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。CST推荐使用100 nM SignalSilence^® Survivin siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Survivin是一个在胎儿发育和肿瘤细胞恶变过程中高表达的抗凋亡蛋白，大小为16 kDa（1）。Survivin结合并抑制caspase-3，通过抑制细胞凋亡调控和促进细胞分裂，从而来控制细胞周期的G2/M-期临界点（2,3）。这个调控过程需要Survivin在P34 CDC2激酶的作用下，在Thr34位发生磷酸化（4）。通过基因打靶实验和Survivin反义RNA实验，应用Thr34磷酸化缺陷的survivin突变体，已经证明了survivin能够抑制肿瘤的生长（5,6）。

1. Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
2. Li, F. et al. (1998) Nature 396, 580-584.
3. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Dog

Specificity / Sensitivity

Survivin siRNA II will inhibit human and dog Survivin expression.

Description

SignalSilence® Survivin siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit survivin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis.

Directions for Use

CST recommends transfection with 100 nM Survivin siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).

1. Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
2. Li, F. et al. (1998) Nature 396, 580-584.
3. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

注意事项：

Storage: Survivin siRNA II is supplied in RNAse-free water.
Aliquot and store at -20°C.