SignalSilence? Stat1 siRNA II

• 产品编号：CST-6544S      品牌：CST       原厂货号：6544S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

CST生产的SignalSilence® Stat1 siRNA II 可以特异性的扰特Stat1的表达，此方法是通过传递双链RNA到细胞中从而选择性的沉默目的基因。所有的SignalSilence® siRNA 产品经过严格的公司内部测试并通过Western验证可有效的降低蛋白的表达。寡聚核苷酸的合成是在三苯甲游基的控制下保持适当的结合效率。寡聚链随后通过亲和固相提纯。退火的双链RNA通过质谱进一步的分析以确定抽提的成分。每一个批次都通过质谱跟前一个批次比较以确定批次间的连贯性。CST推荐转染条件为：转染100 nM Stat1 siRNA II，Stat1蛋白表达量开始降低是在48-72小时后。至于转染的步骤需根据转染试剂的方法手册进行。如有问题请联系我们。Stat1 转录因子的激活是通过一系列的配体完成 (1) ，这对IFN-α和 IFN-γ的响应非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1的形成二聚体, 入核和DNA结合(4)。 Stat1 蛋白是以成对的形式存在——Stat1α (91 kDa) 和 另外的剪切方式Stat1β (84 kDa)。在很多细胞中，这两种形式都是通过IFN-α激活。但是有时 Stat1α是被 IFN-γ激活。 Stat1这种不适当的激活方式在存在于很多肿瘤中(5)。除了酪氨酸磷酸化，Stat1 也能通过p38有丝分裂激活蛋白激酶依赖(MAPK)-dependent的通路在Ser727位点磷酸化而激活，从而响应IFN-α 和其它的细胞胁迫(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

原厂资料：

Description

SignalSilence® Stat1 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Stat1 expression by RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Stat1 siRNA II. Decreased Stat1 expression was observed 48 to 72 hours post-transfection. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

注意事项：

Storage: Stat1 siRNA II is supplied in RNAse-free water. Aliquot
and store at -20ºC.