SignalSilence® PTEN siRNA II 会抑制人类、大鼠、小鼠和猴PTEN的表达。来自Cell Signaling Technology (CST)的SignalSilence®PTEN siRNA II 可以帮助研究者通过RNA干扰特异性地抑制PTEN的表达,这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence®siRNA产品都是经过内部严格检测的,并且通过Western blot 分析证明确实能够减少目的蛋白的表达通过三苯甲基分析每个碱基以监测寡核苷酸的合成,确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较,来保证不同批次之间的最大一致性。CST推荐使用100 nM PTEN siRNA II 进行转染,48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题,请随时联系CST。PTEN(phosphatase and tensin homologue deleted on chromosome ten),也称为MMAC( (mutated in multiple advanced cancers)磷酸酶,是一种存在于多种人类癌症中的肿瘤抑制因子(1)。PTEN编码一个403个氨基酸组成的多肽,最初被认为是一个双特异性蛋白磷酸酶(2)。PTEN的主要底物是3磷酸肌醇激酶(PI3K)激活转化生成的肌醇磷脂(3)。PTEN是PI3K/Akt信号通路的一个主要的负调因子(1,4,5)。PTEN有一个羧基端的非催化调控的结构域,其中含有3个可以调节PTEN的稳定性,也可能影响其生物活性的磷酸化位点(Ser380,Thr382,Thr383)(6,7)。PTEN调节p53蛋白水平和活性(8),并在趋化过程中参与G蛋白偶联信号转导(9,10)。
Species predicted to react based on 100% sequence homology:Mouse, Rat, Monkey
Specificity / Sensitivity
SignalSilence® PTEN siRNA II will inhibit human, mouse, rat and monkey PTEN expression.
Description
SignalSilence® PTEN siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PTEN expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression by western analysis.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
CST recommends transfection with 100 nM PTEN siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Background
PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).