SignalSilence? Bax siRNA II

• 产品编号：CST-6514S      品牌：CST       原厂货号：6514S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® Bax siRNA II 可以帮助研究者通过RNA干扰特异性地抑制 Bax 的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Bax siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Bax 是细胞内通过线粒体胁迫而诱导凋亡过程中一个重要的组分(1)。受到凋亡的刺激后，Bax 形成链式聚合物，并从细胞质转运到线粒体膜上(2)。通过与线粒体膜上的 pore 蛋白互作，Bax 增加了膜的通透性，这使得细胞色素C从线粒体释放，从而激活caspase-9并起始caspase 激活的凋亡信号通路 (3,4)。

1. Wei, M.C. et al. (2001) Science 292, 727-30.
2. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002.
3. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6.
4. Marzo, I. et al. (1998) Science 281, 2027-31.

原厂资料：

Description

SignalSilence® Bax siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bax expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Bax siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).

1. Wei, M.C. et al. (2001) Science 292, 727-30.
2. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002.
3. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6.
4. Marzo, I. et al. (1998) Science 281, 2027-31.

注意事项：

Storage: Bax siRNA II is supplied in RNAse-free water. Aliquot
and store at -20°C.