SignalSilence? Ezh2 siRNA I

• 产品编号：CST-6509S      品牌：CST       原厂货号：6509S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)公司的SignalSilence® Ezh2 siRNA I可以帮助研究者通过RNA干扰特异性地抑制Ezh2的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^®siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST公司推荐使用100 nM Ezh2 siRNA I进行转染。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。polycomb group (PcG)蛋白是涉及维持数个发育调节基因的沉默水平，并且有助于维持细胞身份（cell identity）、细胞周期调节和肿瘤发生(1,2)。zeste homolog 2 (Ezh2)的增强子是一个该蛋白家族成员，它包含四种保守区域包括domain I、domain II、一个半胱氨酸富裕的氨基酸延伸序列和羧基端SET domain (3)。SET domain已经证明与histone methyltransferase (HMTase)活性有关联。此外，哺乳类Ezh2是一个组蛋白去乙酰酶复合物的成员之一，它在基因沉默中有功能，并且在染色质结构水平起着作用(4)。在体内，Ezh2复合物使histone H3在Lys9和27位点甲基化，这被认为是涉及到调节转录因子到特异的靶位点(5)。Ezh2蛋白在不同肿瘤形式中被下调，并且具有主要效应因子和肿瘤发生的调节因子的作用已经变成一个越来越感兴趣的课题(6)。

1. Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
2. Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
3. Chen, H. et al. (1996) Genomics 38, 30-7.
4. Tonini, T. et al. (2004) Oncogene 23, 4930-7.
5. Müller, J. et al. (2002) Cell 111, 197-208.
6. Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.

原厂资料：

Description

SignalSilence® Ezh2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Ezh2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Ezh2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

1. Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
2. Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
3. Chen, H. et al. (1996) Genomics 38, 30-7.
4. Tonini, T. et al. (2004) Oncogene 23, 4930-7.
5. Müller, J. et al. (2002) Cell 111, 197-208.
6. Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.

注意事项：

Storage: Ezh2 siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.