C1QBP (D7H12) XP? Rabbit mAb

• 产品编号：CST-6502S      品牌：CST       原厂货号：6502S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

C1QBP (D7H12) XP® Rabbit mAb 兔单抗能够检测内源性C1QBP总蛋白水平。该单克隆抗体是由合成的人源C1QBP蛋白C末端肽段免疫动物而制备的。C1QBP，又称作p32，p33，gC1q受体（gc1qR）或透明质酸结合蛋白1（HABP1），最初通过与剪切因子（SF-2）的结合被鉴定出来（1）。随后逐渐发现了更多的C1QBP结合蛋白，包括补体c1q的球状头部，透明质酸，几种蛋白激酶（2）以及肿瘤抑制因子（ARF）（3,4,5），多种细菌和病毒抗原（6）。C1QBP在多种类型的肿瘤细胞中高表达，并且可能与Warburg效应有关（肿瘤细胞主要通过糖酵解而不是TCA获取能量）（7）。C1QBP同时还抑制线粒体通透性转换孔（MPT），这可能与对抗氧化应激，保护细胞的机制有关系（8）。

1. Krainer, A.R. et al. (1991) Cell 66, 383-94.
2. Storz, P. et al. (2000) J Biol Chem 275, 24601-7.
3. Itahana, K. and Zhang, Y. (2008) Cancer Cell 13, 542-53.
4. Reef, S. et al. (2007) Oncogene 26, 6677-83.
5. Reef, S. et al. (2006) Mol Cell 22, 463-75.
6. Peerschke, E.I. and Ghebrehiwet, B. (2007) Immunobiology 212, 333-42.
7. Fogal, V. et al. (2010) Mol Cell Biol 30, 1303-18.
8. McGee, A.M. and Baines, C.P. (2010) Biochem J 433, 119-25.

原厂资料：

Specificity / Sensitivity

C1QBP (D7H12) XP® Rabbit mAb recognizes endogenous levels of total C1QBP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human C1QBP protein.

Background

C1QBP, also referred to as p32, p33, gC1q receptor (gC1qR), and hyaluronic acid binding protein 1 (HABP1), was originally identified via its binding interactions with Splicing Factor (SF-2) (1). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases (2), the tumor suppressor ARF (3-5), and multiple antigens of bacterial and viral origin (6). Research studies have shown that C1QBP is overexpressed in a number of cancer cell types (7), and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis (7). C1QBP has also been shown to inhibit the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress (8).

1. Krainer, A.R. et al. (1991) Cell 66, 383-94.
2. Storz, P. et al. (2000) J Biol Chem 275, 24601-7.
3. Itahana, K. and Zhang, Y. (2008) Cancer Cell 13, 542-53.
4. Reef, S. et al. (2007) Oncogene 26, 6677-83.
5. Reef, S. et al. (2006) Mol Cell 22, 463-75.
6. Peerschke, E.I. and Ghebrehiwet, B. (2007) Immunobiology 212, 333-42.
7. Fogal, V. et al. (2010) Mol Cell Biol 30, 1303-18.
8. McGee, A.M. and Baines, C.P. (2010) Biochem J 433, 119-25.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.