SignalSilence? Cofilin siRNA I (Mouse Specific)

• 产品编号：CST-6494S      品牌：CST       原厂货号：6494S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® Cofilin siRNA I (小鼠特异)可以帮助研究者通过RNA干扰特异性地抑制Cofilin的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^®siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^®Cofilin siRNA I (小鼠特异)进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Cofilin和actin-解聚因子（ADF）都属于一组保守的小actin-结合蛋白家族，在胞质分裂，内吞，胚胎发育，压力应激和组织再生过程中发挥了关键作用（1）。在刺激因素作用下，cofilin作为提前存在的细丝促进actin肌动蛋白丝的再生（2）。Cofilin的第3位丝氨酸被LIMK或TESK磷酸化后会抑制cofilin的这种作用（3-5）。第3位丝氨酸磷酸化也会调控cofilin从细胞核转位到细胞质中（6）。

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

原厂资料：

Description

SignalSilence® Cofilin siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Cofilin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® Cofilin siRNA I (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

注意事项：

Storage: Cofilin siRNA I (Mouse Specific) is supplied in
RNAse-free water. Aliquot and store at -20ºC.