SignalSilence? Radixin siRNA I

• 产品编号：CST-6491S      品牌：CST       原厂货号：6491S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

Radixin siRNA I抑制人源、小鼠、大鼠和猴radixin蛋白的表达。Cell Signaling Technology (CST)公司的SignalSilence® Radixin siRNA I允许研究者通过RNA干扰去特异性抑制Radixin蛋白表达，而RNA干扰是通过双链RNA分子传送到细胞中能选择性沉默基因表达的一个方法。所有来自CST公司的SignalSilence® siRNA产品都经过严格的内部检测，且显示通过免疫印迹分析方法减少靶蛋白表达。寡核苷酸的合成是通过三苯基分析（trityl analysis）一个碱基接着一个碱基被监测以确保合适的连接效率。随后通过亲和固相萃取纯化核苷酸。进一步，退火RNA双链通过质谱分析以确保这个双链确切组成。每一批通过质谱分析与前一批比较以确保最大的批间一致。CST公司推荐在细胞裂解前48到72小时转染100 nM Radixin siRNA I。对于转染程序，按照转染试剂商提供的操作步骤。在使用中出现任何问题请随时联系CST公司。ezrin, radixin, and moesin (ERM)蛋白功能作为细胞质膜和肌动蛋白细胞骨架的连接者，并且该蛋白涉及细胞粘附、胞膜边缘波动(membrane ruffling)和微绒毛形成 (1)。ERM蛋白经历分子内或分子间的氨基和羧基端结构域的相互作用，该蛋白是以非活性的细胞基质单体或二聚体存在 (2)。ERM蛋白在羧基端残基(ezrin蛋白Thr567位点、radixin蛋白Thr564位点、moesin蛋白Thr558 位点) 磷酸化可干扰氨基端和羧基端的关联，这可能在调节ERM蛋白的形成和功能中起着重要作用 (3,4)。ezrin蛋白Thr567位点磷酸化对于细胞骨架的重组和致癌基因诱导的转化可能起着关键角色(5)。基于生长因子刺激，Ezrin蛋白也在酪氨酸残基发生磷酸化。在上皮细胞分化期间，ezrin蛋白Tyr353位点的磷酸化传递一个生存信号(6)。

1. Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10.
2. Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92.
3. Matsui, T. et al. (1998) J Cell Biol 140, 647-57.
4. Gautreau, A. et al. (2000) J Cell Biol 150, 193-203.
5. Tran Quang, C. et al. (2000) EMBO J 19, 4565-76.
6. Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Radixin siRNA I inhibits expression of human, mouse, rat, and monkey radixin protein.

Description

SignalSilence® Radixin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit radixin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Background

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

1. Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10.
2. Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92.
3. Matsui, T. et al. (1998) J Cell Biol 140, 647-57.
4. Gautreau, A. et al. (2000) J Cell Biol 150, 193-203.
5. Tran Quang, C. et al. (2000) EMBO J 19, 4565-76.
6. Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5.

注意事项：

Storage: Radixin siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC