PTP4A3 Antibody

• 产品编号：CST-6484S      品牌：CST       原厂货号：6484S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

</p>PTP4A3抗体可以识别内源性总的PTP4A3蛋白。</p> 多抗由合成肽段免疫动物产生，该肽段与人PTP4A3蛋白Gly62邻近氨基酸序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。</p>磷酸酶再生肝（PRL）家族是一组不同的蛋白酪氨酸磷酸酶（PTP），含有一个标志性的磷酸酶结构域而缺少与其他已知PTP蛋白的同源结构。PRL家族现在已知有三种成员（PRL1-3）。PRL-1是第一个被发现的成员，最初是在再生肝脏和有丝分裂原处理的成纤维细胞中发现的一种即早期基因（IEG）（1）。PRL-3，被广泛认为是PTP4A3，由于它在调控细胞增殖中的重要功能以及可能的癌症侵润能力，是PRL家族被最充分表征的成员。由于PRL-家族蛋白特殊的靶向底物尚未被完全认识，最近一个结肠癌细胞系中的研究报道PTP4A3能够去磷酸化integrin β1的Tyr783（2）。PTP4A3也被认为通过抑制docking蛋白p130的酪氨酸磷酸化而在心肌肥大发生过程中发挥了关键作用（3）。永生化细胞系和过表达PTP4A3的小鼠肺肿瘤细胞都能观察到细胞增殖和细胞运动力增加（3,4），而TP4A3蛋白水平的增加与人类肿瘤密切相关（5,6）。

1. Diamond, R.H. et al. (1994) Mol Cell Biol 14, 3752-62.
2. Tian, W. et al. (2012) BMC Biochem 13, 22.
3. Matter, W.F. et al. (2001) Biochem Biophys Res Commun 283, 1061-8.
4. Guo, K. et al. (2004) Cancer Biol Ther 3, 945-51.
5. Pryczynicz, A. et al. (2010) Folia Histochem Cytobiol 48, 632-6.
6. Mayinuer, A. et al. (2013) Ann Surg Oncol 20, 305-17.

原厂资料：

Specificity / Sensitivity

<p>PTP4A3 Antibody recognizes endogenous levels of total PTP4A3 protein.

Source / Purification

<p>Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly62 of human PTP4A3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

<p>The Phosphatase of Regenerating Liver (PRL) family is a distinct group of protein tyrosine phosphatases (PTP), containing a signature phosphatase domain but otherwise lacking homology to known PTP proteins. There are currently three known members of the PRL family (PRL1-3). PRL-1 was the first family member to be identified; it was initially characterized as an immediate early gene (IEG) in regenerating liver and mitogen-treated fibroblasts (1). PRL-3, known widely as PTP4A3, is now the most well-characterized member of the PRL family, due to its important role in regulating cell proliferation, and possibly cancer metastasis. While specific substrates of the PRL-family proteins have remained largely undefined, a recent study in colon cancer cell lines reported that PTP4A3 dephosphorylated integrin β1 at Tyr783 (2). PTP4A3 was also shown to play a potential role in the progression of cardiac hypertrophy by inhibiting tyrosine phosphorylation of the docking protein p130 (3). Increased rates of both cell proliferation and motility have been observed in immortalized cell lines and murine lung tumor cells over-expressing PTP4A3 (3,4), and elevated levels of PTP4A3 protein are associated with a subset of human cancers (5,6).

1. Diamond, R.H. et al. (1994) Mol Cell Biol 14, 3752-62.
2. Tian, W. et al. (2012) BMC Biochem 13, 22.
3. Matter, W.F. et al. (2001) Biochem Biophys Res Commun 283, 1061-8.
4. Guo, K. et al. (2004) Cancer Biol Ther 3, 945-51.
5. Pryczynicz, A. et al. (2010) Folia Histochem Cytobiol 48, 632-6.
6. Mayinuer, A. et al. (2013) Ann Surg Oncol 20, 305-17.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody