SignalSilence? FAK siRNA II

• 产品编号：CST-6483S      品牌：CST       原厂货号：6483S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)公司的 SignalSilence® FAK siRNA II让研究者用RNA 干扰技术特异抑制FAK表达，这是一种通过发送双链RNA分子到细胞选择性抑制基因表达。所有SignalSilence®siRNA产物来自CST，经过严格的内部测试，并由western analysis已经显示减少蛋白表达。核苷酸合成通过三苯甲基单个碱基监测，保证合适的配对效率。寡核苷酸随后由亲和固相抽提法纯化。退火的RNA双螺旋由质谱进一步分析证明双螺旋的准确组成。每一批由质谱比对前一批，以保证最大的批与批之间的一致性。CST 推荐使用100nM FAK siRNA II转染，48到72小时后裂解细胞。对应转染步骤，按转染试剂生产商提供的步骤进行，如在使用上有任何问题请随时联系CST。黏着斑激酶(FAK)是广泛表达的细胞质蛋白酪氨酸激酶，涉及整合素调控的信号转导，它在一些生物过程包括细胞扩散、转移和存活的控制中起重要作用（1）。FAK被整合素聚集而激活，导致在397位酪氨酸的自磷酸化，这是一个Src家族激酶PI3K和PL Cγ的结合位点（2-5）。Src家族激酶的招募导致FAK催化结构域酪氨酸407, 酪氨酸576, and 酪氨酸577的磷酸化，以及FAK的C末端区域871位酪氨酸和925位酪氨酸磷酸化（6,7）。

1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

原厂资料：

Description

SignalSilence® FAK siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit FAK expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM FAK siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

注意事项：

Storage: FAK siRNA II is supplied in RNAse-free water. Aliquot
and store at -20ºC.