SignalSilence? Gelsolin siRNA II

• 产品编号：CST-6473S      品牌：CST       原厂货号：6473S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

Cell Signaling Technology (CST)公司生产的SignalSilence Gelsolin siRNA II使得研究者可以使用RNA干扰技术特异性的抑制gelsolin的表达。这种技术通过将双链RNA分子送入细胞而选择性的沉默某个基因的表达。CST的siRNA产品已经在内部经过严格测试，经western分析确能减少目标蛋白的表达。通过三苯分析对寡核苷酸合成进行逐个碱基的监测，确保了正确的耦联效率。然后用亲和固相萃取纯化寡核苷酸。退火的RNA进一步通过质谱分析，以验证其确切成分。每批都将和以前批次进行质谱比对，以确保批次间最大的一致性。CST公司推荐您在细胞裂解前48-72小时用100nM SignalSilence Gelsolin siRNA II进行转染。转染的流程请遵循转染试剂制造商提供的操作流程。使用中如有任何问题，请与CST联系。Gelsolin（凝溶胶蛋白：肌动蛋白解聚因子，ADF，AGEL，Brevin）是一个83 kDa的蛋白,与villin和adseverin/scinderin具有结构和功能的同源性（1,2）。Gelsolin对肌动蛋白进行加帽，以钙离子依赖的方式切割肌动蛋白，在微丝装配中起重要作用（3,4）。Gelsolin对需要细胞骨架重构的细胞内事件（如膜皱裂、趋化、纤毛发生）是非常重要的（3）；因此，gelsolin敲除的小鼠细胞表现出运动缺陷，包括无法响应生长因子刺激形成皱褶（5,6）。在人类身上，gelsolin蛋白缺陷已与淀粉样型5（AMYL5），一种以颅神经病变为特征的遗传病相关联，它很可能是gelsolin淀粉样沉积的结果（7）。

1. Vitale, M.L. et al. (1991) J Cell Biol 113, 1057-67.
2. Lueck, A. et al. (1998) J Cell Sci 111 ( Pt 24), 3633-43.
3. Sun, H.Q. et al. (1999) J Biol Chem 274, 33179-82.
4. Li, G.H. et al. (2010) Med Res Rev, 32, 999-1025.
5. Azuma, T. et al. (1998) EMBO J 17, 1362-70.
6. Lu, M. et al. (1997) J Cell Biol 138, 1279-87.
7. Haltia, M. et al. (1990) Biochem Biophys Res Commun 167, 927-32.

原厂资料：

Description

SignalSilence® Gelsolin siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit gelsolin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® Gelsolin siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Gelsolin (actin-depolymerizing factor, ADF, AGEL, Brevin) is an 83 kDa protein that shares structural and functional homology to villin and adseverin/scinderin (1,2). Gelsolin plays an important role in actin filament assembly by capping and severing actin proteins in a Ca2+-dependent manner (3,4). Gelsolin is important for cellular events (e.g., membrane ruffling, chemotaxis, ciliogenesis) that require cytoskeletal remodeling (3); accordingly, cells from gelsolin knockout mice exhibit motility defects, including a failure to ruffle in response to growth factor stimulation (5,6). In humans, defects in gelsolin have been linked to amyloidosis type 5 (AMYL5), a hereditary disease characterized by cranial neuropathy, which appears to result from gelsolin amyloid deposition (7).

1. Vitale, M.L. et al. (1991) J Cell Biol 113, 1057-67.
2. Lueck, A. et al. (1998) J Cell Sci 111 ( Pt 24), 3633-43.
3. Sun, H.Q. et al. (1999) J Biol Chem 274, 33179-82.
4. Li, G.H. et al. (2010) Med Res Rev, 32, 999-1025.
5. Azuma, T. et al. (1998) EMBO J 17, 1362-70.
6. Lu, M. et al. (1997) J Cell Biol 138, 1279-87.
7. Haltia, M. et al. (1990) Biochem Biophys Res Commun 167, 927-32.

注意事项：

Storage: Gelsolin siRNA II is supplied in RNAse-free water.
Aliquot and store at -20ºC.