SignalSilence? Caspase-3 siRNA I

• 产品编号：CST-6466S      品牌：CST       原厂货号：6466S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® Caspase-3 siRNA I可以帮助研究者通过RNA干扰特异性地抑制Caspase-3的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。CST推荐使用100 nM SignalSilence^® Caspase-3 siRNA I 进行转染，48到72小时后对细胞进行裂解能检测到Caspase-3的下调表达。转染步骤按照转染试剂说明书提供的步骤进行。Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者，因为它对许多关键的蛋白起着部分或全部的裂解作用，比如核酶多聚(ADP-ribose)聚合酶(PARP)（1）。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点（2）。除了在细胞凋亡中的关键作用外，经RNA干扰技术证实Caspase-3在红细胞分化中发挥作用（3）。

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.
3. Carlile, G. W. et al. (2004) Blood 103, 4310-4316.

原厂资料：

Description

SignalSilence® Caspase-3 siRNA allows the researcher to specifically inhibit Caspase-3 expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.

Directions for Use

CST recommends transfection with 100 nM Caspase-3 siRNA. Decreased Caspase-3 expression was observed 24-72 hours post-transfection. See Protocol for transfection procedure.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).In addition to its central role in apoptosis, Caspase-3 has been shown to play a role in erythroid differentiation through the use of RNA interference (3).

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.
3. Carlile, G. W. et al. (2004) Blood 103, 4310-4316.

注意事项：

Storage: Caspase-3 siRNA is supplied in
RNase-free water. Aliquot and store at –20°C.