DR1 Antibody

• 产品编号：CST-6447S      品牌：CST       原厂货号：6447S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DR1 Antibody能够检测内源性DR1总蛋白水平。通过人工合成人源DR1蛋白Gly112位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Down-regulator of transcription 1 (DR1)也称为负性辅助因子2-β (NC2-β)，该蛋白可以与DR1 associated protein 1 (DRAP1)/NC2-α形成一个异源二聚体，并且作为RNA polymerase II和III (RNAPII和III)的一个负性调节因子(1-5)。DR1活性被认为对于在基本的转录活性和转录激活因子驱动转录之间开关的修饰中起着重要作用 (2,6,7)。DR1蛋白与 TATA binding protein (TBP)的相互作用封闭了一般转录因子TFIIA和TFIIB与TBP的关联，并且干扰RNAPII转录起始复合物的形成(1,8,9)。RNAPIII驱动的转录也能通过DR1与TBP相互作用来被抑制。DR1蛋白干扰TBP与TFIIB related factor (BRF)/RNAPIII B-related factor相互作用，这就通过RNAPIII蛋白抑制转录起始(4)。

1. Inostroza, J.A. et al. (1992) Cell 70, 477-89.
2. Meisterernst, M. and Roeder, R.G. (1991) Cell 67, 557-67.
3. Mermelstein, F. et al. (1996) Genes Dev 10, 1033-48.
4. White, R.J. et al. (1994) Science 266, 448-50.
5. Kantidakis, T. and White, R.J. (2010) Nucleic Acids Res 38, 1228-39.
6. Kraus, V.B. et al. (1994) Proc Natl Acad Sci U S A 91, 6279-82.
7. Yeung, K.C. et al. (1994) Genes Dev 8, 2097-109.
8. Kim, T.K. et al. (1995) J Biol Chem 270, 10976-81.
9. Kamada, K. et al. (2001) Cell 106, 71-81.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: D. melanogaster, Zebrafish, Dog, Pig

Specificity / Sensitivity

DR1 Antibody recognizes endogenous levels of total DR1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human DR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Down-regulator of transcription 1 (DR1), also known as negative cofactor 2-β (NC2-β), forms a heterodimer with DR1 associated protein 1 (DRAP1)/NC2-α and acts as a negative regulator of RNA polymerase II and III (RNAPII and III) transcription (1-5). DR1 activity is thought to be important for modulating the switch between basal transcription activity and transcription activator driven transcription (2,6,7). DR1 interaction with TATA binding protein (TBP) blocks the association of general transcription factors TFIIA and TFIIB with TBP and disrupts the formation of the RNAPII transcription initiation complex (1,8,9). RNAPIII driven transcription is also inhibited by DR1 interaction with TBP. DR1 disrupts the interaction of TBP with the TFIIB related factor (BRF)/RNAPIII B-related factor, inhibiting transcription initiation by the RNAPIII machinery (4).

1. Inostroza, J.A. et al. (1992) Cell 70, 477-89.
2. Meisterernst, M. and Roeder, R.G. (1991) Cell 67, 557-67.
3. Mermelstein, F. et al. (1996) Genes Dev 10, 1033-48.
4. White, R.J. et al. (1994) Science 266, 448-50.
5. Kantidakis, T. and White, R.J. (2010) Nucleic Acids Res 38, 1228-39.
6. Kraus, V.B. et al. (1994) Proc Natl Acad Sci U S A 91, 6279-82.
7. Yeung, K.C. et al. (1994) Genes Dev 8, 2097-109.
8. Kim, T.K. et al. (1995) J Biol Chem 270, 10976-81.
9. Kamada, K. et al. (2001) Cell 106, 71-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.