SignalSilence? XIAP siRNA I

• 产品编号：CST-6446S      品牌：CST       原厂货号：6446S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® XIAP siRNA I 可以帮助研究者通过RNA干扰特异性地抑制XIAP的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。CST推荐使用100 nM SignalSilence^® XIAP siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。凋亡抑制因子蛋白(IAP) 家族包含一个进化上保守的凋亡抑制分支，此分支蛋白包含70个氨基酸BIR (杆状病毒抑制重复) 域(1,2)。在人类中，此家族的蛋白包含c-IAP1，c-IAP2，XIAP，survivin，livin 和NAIP。IAP 家族成员在癌细胞系和原癌中过表达，尤其是survivin和livin, 表明这些蛋白在癌症发展过程中起到了重要作用(3-5)。一般来讲，IAP蛋白是通过直接抑制几个caspases的活性发挥作用, 包括caspase-3、caspase-7和caspase-9 (5,6)。另外，IAP家族的成员与线粒体蛋白Smac的结合，阻止了它们与caspase-9的互作，因此引起了caspase的加工及其激活 (2)。利用RNA干扰技术，研究证实XIAP具有通过MURR1调节铜稳态的功能（7）。

1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
7. Burstein, E. et al. (2004) EMBO J 23, 244-54.

原厂资料：

Description

SignalSilence® XIAP siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit XIAP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Directions for Use

CST recommends transfection with 100 nM XIAP siRNA I. Decreased XIAP expression was seen 24-72 hours post-transfection. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).Using RNA intereference, XIAP has been shown to function through MURR1 to regulate copper homeostasis (7).

1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
7. Burstein, E. et al. (2004) EMBO J 23, 244-54.

注意事项：

Storage: XIAP siRNA is supplied in RNase-
free water. Aliquot and store at –20°C.