SignalSilence? Bcl-2 siRNA I

• 产品编号：CST-6441S      品牌：CST       原厂货号：6441S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® Bcl-2 siRNA I 能够抑制人源、鼠源和大鼠源 Bcl-2 的表达。来自Cell Signaling Technology (CST)的SignalSilence^® Bcl-2 siRNA I 可以帮助研究者通过RNA干扰特异性地抑制 Bcl-2 的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Bcl-2 siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Bcl-2 通过抑制线粒体细胞色素C的释放而对一系列的凋亡因素做出响应，从而发挥其促进细胞存活的功能(1)。这提示其在调节线粒体钙离子的动态平衡和质子流方面发挥着作用(2)。在Bcl-2中已经发现了几个磷酸化位点，包括Thr56, Ser70, Thr74 和 Ser87 (3)。这些磷酸化位点可能是ASK1/MKK7/JNK1通路的靶点,并且Bcl-2的磷酸化可能是有丝分裂活动的一个标志(4,5)。在T淋巴细胞糖（肾上腺）皮质激素诱导的凋亡过程中，Bcl-2在Thr56或者Ser87位点的突变能够抑制其抗凋亡的活性(6)。白细胞介素3 和JNK诱导的Bcl-2在Ser70 位点的磷酸化可能是其增强抗凋亡作用所必需的(7)。通过RNA干扰将Bcl-2的表达沉默，诱导了 p53 依赖的凋亡(8)。

1. Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.
2. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.
3. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.
4. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.
5. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.
6. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.
7. Deng, X. et al. (2001) J Biol Chem 276, 23681-8.
8. Jiang, M. and Milner, J. (2003) Genes Dev 17, 832-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

SignalSilence^® Bcl-2 siRNA I will inhibit human, mouse and rat Bcl-2 expression.

Description

SignalSilence^® Bcl-2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bcl-2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® Bcl-2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).Silencing Bcl-2 expression by RNA interference induces p53 dependent apoptosis (8).

1. Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.
2. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.
3. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.
4. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.
5. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.
6. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.
7. Deng, X. et al. (2001) J Biol Chem 276, 23681-8.
8. Jiang, M. and Milner, J. (2003) Genes Dev 17, 832-7.

注意事项：

Storage: Bcl-2 siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.