SignalSilence? CK2α siRNA I

• 产品编号：CST-6389S      品牌：CST       原厂货号：6389S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

研究人员可以使用CST的SignalSilence® CK2α siRNA I，采用RNA干扰（一种通过传递外源性的双链RNA分子到细胞中从而能选择性的沉默基因表达的方法）技术特异性的抑制CK2α蛋白表达。CST所有的SignalSilence® siRNA产品都经过了严格的内部测试并采用Western分析证明其可以有效降低靶蛋白的表达。为了保证适当的结合效率，我们采用三苯甲基分析的方法逐个碱基的监测寡核苷酸的合成。随后通过亲和固相提取纯化寡核苷酸。退火的双链RNA进一步通过质谱分析来验证双链复合体的确切成分。每一批产品和前一批都要通过质谱分析进行比较以确定批次间最大限度的一致性。CST建议使用100 nM CK2α siRNA I转染48-72小时后裂解细胞。转染的具体步骤请参考转染试剂制造商提供的操作标准。使用过程中有任何问题请随时联系CST。 CK2(以前称作酪蛋白激酶 II)是一种高度保守的具有300多个作用底物的蛋白激酶，能够调节细胞生长、细胞死亡和细胞存活。CK2在响应紫外照射诱导的DNA损伤中被发现，它能够靶向XRCC1 (1) 和BRCA1 (2)并能调节p53肿瘤抑制基因的功能(3)。此外，CK2在NF-κB的激活过程中发挥着关键的作用(4)。紫外照射能够刺激CK2介导的IκBα中几个羧基末端残基的磷酸化，引起IκBα的蛋白酶体降解和释放以及活化的NF-κB的核转移。CK2在许多肿瘤和神经退行性疾病如阿尔茨海默氏症和帕金森症中功能紊乱(6)。结构上讲，CK2是一个多聚化的蛋白复合体，由两个催化亚基(α or α')和两个调节亚基β组成(7)。CK2分布广泛，明显的组成活跃(7)。尽管CK2α 和CK2β上已鉴定出细胞周期依赖性的Ser-Pro磷酸化位点，Src-相关激酶 c-Fgr作用的255位酪氨酸磷酸化似乎对CK2α的活性有最大的影响（8,9)。

1. Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24.
2. O'Brien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64.
3. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71.
4. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
5. Trembley, J.H. et al. Biofactors 36, 187-95.
6. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print.
7. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8.
8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9.
9. Litchfield, D.W. (2003) Biochem J 369, 1-15.

原厂资料：

Description

SignalSilence® CK2α siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CK2α expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM CK2α siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 

Background

CK2 (formerly called Casein Kinase II) is a highly conserved protein kinase with more than 300 substrates regulating cell growth, cell death, and cell survival. CK2 has been implicated in the response to UV irradiation-induced DNA damage, targeting XRCC1 (1) and BRCA1 (2) as well as regulating p53 tumor suppressor protein functions (3). Furthermore, CK2 plays a key role in NF-κB activation (4). UV irradiation stimulates CK2-mediated phosphorylation of several carboxy-terminal residues within IκBα, resulting in IκBα proteasomal degradation and the release and nuclear translocation of active NF-κB. CK2 is also dysregulated in many cancers (5) and neurodegenerative diseases such as Alzheimer's and Parkinson's diseases (6). Structurally, CK2 is a multimeric protein complex consisting of two catalytic subunits (α or α') and two regulatory β subunits (7). CK2 is distributed ubiquitously and is apparently constitutively active (7). While cell cycle-dependent Ser-Pro phosphorylation sites have been identified on CK2α and CK2β, Tyr255 phosphorylation by the Src-related kinase c-Fgr seems to have the greatest effect on CK2α activity (8,9). 

1. Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24.
2. O'Brien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64.
3. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71.
4. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
5. Trembley, J.H. et al. Biofactors 36, 187-95.
6. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print.
7. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8.
8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9.
9. Litchfield, D.W. (2003) Biochem J 369, 1-15.

注意事项：

Storage: CK2α siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.