SignalSilence? Bcl-xL siRNA II

• 产品编号：CST-6363S      品牌：CST       原厂货号：6363S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® Bcl-xL siRNA II 可以帮助研究者通过RNA干扰特异性地抑制 Bcl-xL 的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Bcl-xL siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。Bcl-xL 通过两种不同的机制来阻止凋亡：通过与凋亡蛋白形成异源二聚体来阻止其凋亡作用(1,2)。并且可形成线粒体膜外微孔来帮助在胁迫环境下保持正常的膜状态(3)。Bcl-xL 在经微管破坏物如paclitaxel、vinblastine 和nocodazole处理后能够被JNK磷酸化 (4,5)。

1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.

原厂资料：

Description

SignalSilence® Bcl-xL siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bcl-xL expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Bcl-xL siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.

注意事项：

Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.