SignalSilence? Stat6 siRNA I

• 产品编号：CST-6358S      品牌：CST       原厂货号：6358S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

CST生产的SignalSilence® Stat6 siRNA I可以通过RNA干异性的扰特Stat6的表达，此方法是通过传递双链RNA到细胞中从而选择性的沉默目的基因。所有的SignalSilence® siRNA 产品经过严格的公司内部测试并通过Western blot验证可有效的降低蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐转染条件为100 nM Stat6 siRNA I，48-72小时后裂解细胞。转染步骤需根据转染试剂的方法手册进行。如有问题请联系我们。受到Janus激酶激活后, Stat6 入核并调控细胞素诱导基因的表达。Stat6 通过在Tyr641位点的磷酸化激活，这对IL-4 和 IL-13的应答是必需的(1-4)。另外， 在B细胞系中Stat6被 IFN-α 激活并与 Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶 2A 也参与到 IL-4介导的 Stat6信号通路中 (7)。

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

原厂资料：

Description

SignalSilence® Stat6 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Stat6 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Stat6 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

注意事项：

Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.