SignalSilence? Bax siRNA I

• 产品编号：CST-6321S      品牌：CST       原厂货号：6321S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® Bax siRNA I 可以帮助研究者通过RNA干扰特异性地抑制 Bax 的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。CST推荐使用100 nM SignalSilence^® Bax siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。Bax 是细胞内通过线粒体胁迫而诱导凋亡过程中一个重要的组分(1)。受到凋亡的刺激后，Bax 形成链式聚合物，并从细胞质转运到线粒体膜上(2)。通过与线粒体膜上的 pore 蛋白互作，Bax 增加了膜的通透性，这使得细胞色素C从线粒体释放，从而激活caspase-9并起始caspase 激活的凋亡信号通路 (3,4)。通过RNA干扰实验，Bax 在T淋巴细胞中的表达明显下降，这证明了在凋亡的过程中依赖于Bax引起的AIF从线粒体释放(5)。

1. Wei, M.C. et al. (2001) Science 292, 727-30.
2. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002.
3. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6.
4. Marzo, I. et al. (1998) Science 281, 2027-31.
5. Bidere, N. et al. (2003) J. Biol. Chem. 278(33) , 31401-31411.

原厂资料：

Description

SignalSilence® Bax siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bax expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Directions for Use

CST recommends transfection with 100 nM Bax siRNA. Decreased Bax expression seen 24 hours post-transfection. See Protocol for transfection procedure.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).Bax expression was effectively reduced in T lymphocytes by RNA interference, demonstrating the dependence on Bax to trigger the release of AIF from the mitochondria during apoptosis (5).

1. Wei, M.C. et al. (2001) Science 292, 727-30.
2. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002.
3. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6.
4. Marzo, I. et al. (1998) Science 281, 2027-31.
5. Bidere, N. et al. (2003) J. Biol. Chem. 278(33) , 31401-31411.

注意事项：

Storage: Bax siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.