SignalSilence? Merlin siRNA I

• 产品编号：CST-6316S      品牌：CST       原厂货号：6316S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

Cell Signaling Technology (CST)公司生产的SignalSilence® Merlin siRNA I使得研究者可以使用RNA干扰技术特异性的抑制Merlin的表达。这种技术通过将双链RNA分子送入细胞而选择性的沉默某个基因的表达。所有SignalSilence® CST的siRNA产品已经在内部经过严格测试，经western分析确能减少目标蛋白的表达。通过三苯分析对寡核苷酸合成进行逐个碱基的监测，确保了正确的耦联效率。然后用亲和固相萃取纯化寡核苷酸。退火的RNA进一步通过质谱分析，以验证其确切成分。每批都将和以前批次进行质谱比对，以确保批次间最大的一致性。CST公司推荐您在细胞裂解前用100nM MerlinK5 siRNA I进行48-72小时的转染。转染的流程请遵循转染试剂制造商提供的操作流程。使用中如有任何问题，请与CST联系。神经纤维瘤病2（NF2）是一种常染色体显性遗传病，以前庭神经鞘瘤，脑膜瘤和其他神经系统肿瘤的发生为特点。NF2家族性肿瘤患者和人群中散发的肿瘤，都是由NF2肿瘤抑制基因的失活引起。Merlin (moesin, ezrin和radixin样蛋白)都是NF2基因的产物，与ezrin, radixin和moesin (ERM)有惊人的相似性。merlin (aka schwannomin) 和 ERM蛋白的调控涉及家族成员中分子内部及分子间的头尾关系（1）。Merlin和ERM蛋白的功能是作为细胞膜和细胞骨架之间的连接，影响细胞的形态，极性和信号转导（2）。Rac/Cdc42的效应器p21激活的激酶（PAK）可以磷酸化Merlin的Ser518，负向调节Rac（3,4）。

1. Ramesh, V. (2004) Nat. Rev. Neurosci. 5, 462-470.
2. Bretscher, A. et al. (2002) Nat. Rev. Mol. Cell Biol. 3, 586-599.
3. Xiao, G. H. et al. (2002) J. Biol. Chem. 277, 883-886.
4. Kissil, J. L. et al. (2003) Mol. Cell 12, 841-849.

原厂资料：

Description

SignalSilence® Merlin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Merlin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Merlin siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Neurofibromatosis 2 (NF2) is an autosomal dominant inherited disorder that is characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors in the general population are caused by inactivation of the NF2 tumor suppressor gene. Merlin (moesin, ezrin, and radixin like protein), the NF2 gene product, has striking similarity to ezrin, radixin, and moesin (ERM). Regulation of merlin (aka schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4).

1. Ramesh, V. (2004) Nat. Rev. Neurosci. 5, 462-470.
2. Bretscher, A. et al. (2002) Nat. Rev. Mol. Cell Biol. 3, 586-599.
3. Xiao, G. H. et al. (2002) J. Biol. Chem. 277, 883-886.
4. Kissil, J. L. et al. (2003) Mol. Cell 12, 841-849.

注意事项：

Storage: Merlin siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC.