SignalSilence? PDK1 siRNA I

• 产品编号：CST-6314S      品牌：CST       原厂货号：6314S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

CST公司的 SignalSilence ® PDK1 siRNA I使得研究者可以通过使用ＲＮＡ干扰来特异性抑制PDK1的表达。RNA干扰是一种通过向细胞中输入双链RNA分子来选择性的抑制基因的表达。CST公司的所有SignalSilence ® siRNA 产品已经过严格的内部测试，已经过western 分析验证可以降低靶蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST建议用100 nM PDK1 siRNA I转染48-72 小时后裂解细胞。对于转染步骤，转染试剂生产商已经提供。如果有问题请与ＣＳＴ联系。磷酸肌醇依赖蛋白激酶1（PDK1）可以在多种信号传导通路中发挥作用（1,2），包括Akt和PKC同工酶p70 S6激酶和RSK的活化（3）。通过这些激酶，PDK1参与调控了广泛的生物学过程，包括细胞增殖，分化和凋亡。

1. Belham, C. et al. (1999) Curr. Biol. 9, R93-R96.
2. Toker, A. and Newton, A.C. (2000) Cell 103, 185-188.
3. Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448.

原厂资料：

Description

SignalSilence® PDK1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PDK1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM PDK1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

1. Belham, C. et al. (1999) Curr. Biol. 9, R93-R96.
2. Toker, A. and Newton, A.C. (2000) Cell 103, 185-188.
3. Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448.

注意事项：

Storage: PDK1 siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC