SignalSilence? eIF4E siRNA I

• 产品编号：CST-6311S      品牌：CST       原厂货号：6311S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

siRNA I能抑制人源和大鼠eIF4E蛋白表达.Cell Signaling Technology (CST)公司的SignalSilence® eIF4E siRNA I允许研究者通过RNA干扰去特异性抑制eIF4E蛋白表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。CST公司推荐在细胞裂解前48到72小时转染100 nM SignalSilence® eIF4E siRNA I。Eukaryotic initiation factor 4E (eIF4E)结合mRNA帽子结构从而介导翻译的起始(1,2)。eIF4E和eIF4G相互作用，而eIF4G是一个支架蛋白其能促进eIF4E和eIF4A装配到eIF4F复合物上(2)。eIF4B被认为在翻译起始阶段有助于eIF4F复合物的形成。在有丝分裂和Erk、p38 MAPK介导的压力刺激下，体内Mnk1蛋白使eIF4E蛋白在Ser209位点磷酸化(3,4)。在小鼠Mnk1上的两个Erk和p38 MAPK磷酸化位点(Thr197 and Thr202)对Mnk1激酶活性至关重要(3)。eIF4G蛋白羧基末端含有血清刺激的磷酸化位点，包括Ser1108、Ser1148和Ser1192 (5)。通过PI3激酶抑制剂LY294002和FRAP/mTOR通路抑制剂rapamycin可阻断这些位点的磷酸化。

1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
5. Raught, B. et al. (2000) EMBO J. 19, 434-444.
6. Yamagiwa , Y. et al. (2003) Hepatology 38, 158-166.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

eIF4E siRNA I will inhibit human and rat eIF4E expression.

Description

SignalSilence® eIF4E siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit eIF4E expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® eIF4E siRNA I 48-72 hours prior to cell lysis.

Background

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
5. Raught, B. et al. (2000) EMBO J. 19, 434-444.
6. Yamagiwa , Y. et al. (2003) Hepatology 38, 158-166.

注意事项：

Storage: eIF4E siRNA I is supplied in RNAse-free water. Aliquot
and store at -20°C.