SignalSilence? GSK-3α/β siRNA

• 产品编号：CST-6301S      品牌：CST       原厂货号：6301S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

GSK-3α/β siRNA可以抑制人，小鼠和大鼠的GSK-3α/β表达。CST公司的 SignalSilence ® GSK-3α/β siRNA使得研究者可以通过使用ＲＮＡ干扰来特异性抑制GSK-3α 和GSK-3β的表达。这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST建议用50 nM GSK-3α/β siRNA转染48-72 小时后裂解细胞。请参考转染步骤。

糖原合酶激酶-3（GSK-3）最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶（1）。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶，可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子，其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制（2,3）。GSK-3不仅参与盘基网柄菌细胞命运的调控，而且是果蝇，爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分（4）。GSK-3还参与调节Cyclin D1 蛋白降解和亚细胞定位（5）。

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
6. Yu, J. Y. et al. (2003) Mol. Ther. 7, 228-236.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

GSK-3α/β siRNA will inhibit human, mouse and rat GSK-3α/β expression.

Description

SignalSilence® GSK-3α/β siRNA from Cell Signaling Technology allows the researcher to specifically inhibit GSK-3aα and GSK-3bβ expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 50 nM GSK-3alpha/beta siRNA 48 hours prior to cell lysis. See Protocol for transfection procedure.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Small Interfering RNA (siRNA) has been used to specifically silence GSK-3alpha and beta expression, causing an upregulation of beta-catenin protein levels (6).

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
6. Yu, J. Y. et al. (2003) Mol. Ther. 7, 228-236.

注意事项：

Storage: GSK-3α/β siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC.