SignalSilence? MARK2 siRNA I

• 产品编号：CST-6266S      品牌：CST       原厂货号：6266S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

来自Cell Signaling Technology (CST)的SignalSilence^® MARK2 siRNA I可以帮助研究者通过RNA干扰特异性地抑制MARK2的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^®MARK2 siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。微管相关蛋白调节微管的稳定性和控制相关进程例如细胞极化/分化、轴突生长、细胞分裂和细胞器的运输(1)。丝氨酸/苏氨酸激酶的MARK (MAP/microtubule affinity-regulating kinases)家族(MARK1-4)被鉴定基于它们的有能力去磷酸化microtubule-associated proteins (MAPs)包括tau、MAP2和MAP4蛋白(2-6)。MARK蛋白在它们的微管结合区域里磷酸化MAPs，这就引起MAPs蛋白从微管上分离，并且增加了微管的动力(2-4)。至于tau蛋白，在阿尔茨海默病人中观察其磷酸化已经被假设有助于神经纤维缠结的形成。MARK激酶的过表达导致MAPs的过磷酸化 、形态学改变和细胞死亡(4)。肿瘤抑制激酶LKB1磷酸化MARK激酶和紧密相关的含有T-loops的AMP-kinases ，导致活性的增加(7)。

1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
2. Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
3. Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
4. Drewes, G. et al. (1997) Cell 89, 297-308.
5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
6. Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
7. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.

原厂资料：

Description

SignalSilence® MARK2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit MARK2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM MARK2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7).

1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
2. Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
3. Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
4. Drewes, G. et al. (1997) Cell 89, 297-308.
5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
6. Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
7. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.

注意事项：

Storage: MARK2 siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC