SignalSilence? Axl siRNA I

• 产品编号：CST-6263S      品牌：CST       原厂货号：6263S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

CST公司的SignalSilence® Axl siRNA I能够通过RNA干扰的方法特异阻断Axl的表达，本方法通过向细胞内输入双链RNA选择性地对基因表达进行沉默。所有的SignalSilence® siRNA产品均经过严格的内部测试并在免疫印迹检测中显示能够降低靶蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST建议使用100 nM Axl siRNA I转染48-72小时后裂解细胞。转染的具体步骤请参考转染试剂制造商提供的操作标准。使用过程中有任何问题请随时联系CST。Axl、 Sky和 Mer是 受体酪氨酸激酶 (RTK) 家族的三个成员，它们有共同的胞内酪氨酸激酶区域和与胞外粘附分子相似的胞外区。这些受体酪氨酸激酶与维生素K依赖性的生长停滞特异性6蛋白（Gas6）结合，Gas6蛋白与S抗凝血因子蛋白结构上相关(1)。与受体结合后，Gas6能够激活PI3K激酶和它的下游靶分子Akt、 S6K和 NF-κB (2,3)。大量证据表明Gas6/Axl信号通路与正常细胞和癌细胞的生长和存活相关(4)。

1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.

原厂资料：

Description

SignalSilence® Axl siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Axl expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM Axl siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Axl, Sky and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.

注意事项：

Storage: Axl siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.