SignalSilence? PTEN siRNA I

• 产品编号：CST-6251S      品牌：CST       原厂货号：6251S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence® PTEN siRNA I 能够抑制人、小鼠、大鼠和猴子PTEN的表达。来自Cell Signaling Technology (CST)的SignalSilence^® PTEN siRNA I 可以帮助研究者通过RNA干扰特异性地抑制PTEN的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM PTEN siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。PTEN（10号染色体上敲除同源性磷酸酶和张力蛋白），又名MMAC磷酸酶（突变多发性晚期癌症），在人类肿瘤中的一种抑癌基因（1）。PTEN编码包含403个氨基酸的多肽，是一种具有双特异性的蛋白磷酸酶（2）。PTEN的主要底物是肌醇磷脂，是由3-磷酸肌醇（PI3K）产生的（3）。PTEN在PI3K/Akt 信号通路中是一种负调控因子（1,4,5）。PTEN包含一个氨基末端，未催化的调节域，此调节域带有3个磷酸化位点（ Ser380, Thr382, 和Thr383 ）来调节PTEN的稳定性，可能影响其生物活性（6,7）。PTEN可以调节p53的水平和活性（8），参与趋化过程中G蛋白偶联信号（9,10）

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

SignalSilence® PTEN siRNA I will inhibit human, mouse, rat and monkey PTEN expression.

Description

SignalSilence® PTEN siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PTEN expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Background

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

1. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
2. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
3. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
4. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
5. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
6. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
7. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
9. Funamoto, S. et al. (2002) Cell 109, 611-23.
10. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

注意事项：

Storage: PTEN siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.