MYH (D13D4) Rabbit mAb

• 产品编号：CST-6248S      品牌：CST       原厂货号：6248S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 MYH (D13D4) Rabbit mAb 能够检测内源性MYH总蛋白。该单克隆抗体是由合成的人源的针对MYH蛋白羧基末端的肽段免疫动物生产的。碱基切除修复(BER)蛋白能够催化清除DNA中错误的或损坏的碱基，包括被氧化的碱基。BER的第一步是针对特定损伤特异的N-糖基酶清除错误的碱基。MYH是大肠杆菌MutY(一种DNAN-糖基酶，可以催化清除8-O G:A错配)的哺乳动物直接同源蛋白(1)。在人类细胞中已检测到几个分布于细胞核或线粒体的MYH亚型(2)。MYH可以和DNA修复蛋白相互作用并集中在氧化损伤后的DNA损伤位点(3)。相应的MYH基因突变和人类胃癌(4)和结肠癌(5-7)有关。

1. Slupska, M.M. et al. (1996) J Bacteriol 178, 3885-92.
2. Ohtsubo, T. et al. (2000) Nucleic Acids Res 28, 1355-64.
3. Shi, G. et al. (2006) Biochem J 400, 53-62.
4. Kobayashi, K. et al. (2008) Anticancer Res 28, 215-21.
5. Bai, H. et al. (2007) Cancer Lett 250, 74-81.
6. Pope, M.A. et al. (2005) DNA Repair (Amst) 4, 315-25.
7. Wooden, S.H. et al. (2004) Cancer Lett 205, 89-95.

原厂资料：

Specificity / Sensitivity

MYH (D13D4) Rabbit mAb recognizes endogenous levels of total MYH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MYH protein. 

Background

Base excision repair (BER) proteins catalyze the removal of incorrect or damaged bases, including oxidized bases, from DNA. N-glycosylases specific to a given lesion remove the incorrect base as the first step in BER. MYH is the mammalian ortholog of E. coli MutY, a DNA glycosylase that catalyzes the removal of 8-oxoG:A mismatches (1). Several MYH isoforms have been detected in human cells localizing to either the nucleus or the mitochondria (2). MYH interacts with DNA repair proteins and localizes to DNA damage foci after oxidative damage (3). Research studies have shown that mutations in the corresponding MYH gene are associated with human gastric (4) and colorectal (5-7) cancers.

1. Slupska, M.M. et al. (1996) J Bacteriol 178, 3885-92.
2. Ohtsubo, T. et al. (2000) Nucleic Acids Res 28, 1355-64.
3. Shi, G. et al. (2006) Biochem J 400, 53-62.
4. Kobayashi, K. et al. (2008) Anticancer Res 28, 215-21.
5. Bai, H. et al. (2007) Cancer Lett 250, 74-81.
6. Pope, M.A. et al. (2005) DNA Repair (Amst) 4, 315-25.
7. Wooden, S.H. et al. (2004) Cancer Lett 205, 89-95.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.